PASADENA hyperpolarization of 13C biomolecules: equipment design and installation
PASADENA hyperpolarization of 13C biomolecules: equipment design and installation
复制标题
DOI：
10.1007/s10334-008-0155-x
复制
发表时间：
2009-04-01
影响因子：
2.3
通讯作者：
Bhattacharya, Pratip
中科院分区：
文献类型：
--
作者：
Hoevener, Jan-Bernd;Chekmenev, Eduard Y.;Bhattacharya, Pratip
PASADENA方法在与体内MRI和mrs相关的生物分子液体样品中实现了16-20%的超极化（在4.7 T下超过40,000倍的信号增强）。然而，目前还没有商用设备可以在常规基础上方便且可重复地进行该实验，以将PASADENA转化为生物医学重要性问题。本论文描述了设计用于每小时快速生产6至8个液体样品的设备，具有高的超偏振再现性。根据一个早期但未发表的原型，我们提供了一个传递电路的示意图，一个层流反应室在一个紧凑的，可移动的外壳中包含一个低场核磁共振。组装指令提供了计算机驱动的，半自动的帕萨迪纳偏光片可以构造。与一个可用的准氢发生器一起，偏振器可以由一个研究者操作，每52秒完成一个超偏振周期。提出了有效性的证据。与竞争对手相比，商业上可用的动态核极化设备的特点是每周期需要90分钟，PASADENA提供了高通量的低成本替代方案。该设备适用于已建立小动物核磁共振并希望探索异核((13)C和（15）N) MRI， MRS的潜力的研究人员，它利用超极化提供的巨大灵敏度增益。
The PASADENA method has achieved hyperpolarization of 16-20% (exceeding 40,000-fold signal enhancement at 4.7 T), in liquid samples of biological molecules relevant to in vivo MRI and MRS. However, there exists no commercial apparatus to perform this experiment conveniently and reproducibly on the routine basis necessary for translation of PASADENA to questions of biomedical importance. The present paper describes equipment designed for rapid production of six to eight liquid samples per hour with high reproducibility of hyperpolarization.Drawing on an earlier, but unpublished, prototype, we provide diagrams of a delivery circuit, a laminar-flow reaction chamber within a low field NMR contained in a compact, movable housing. Assembly instructions are provided from which a computer driven, semi-automated PASADENA polarizer can be constructed.Together with an available parahydrogen generator, the polarizer, which can be operated by a single investigator, completes one cycle of hyperpolarization each 52 s. Evidence of efficacy is presented. In contrast to competing, commercially available devices for dynamic nuclear polarization which characteristically require 90 min per cycle, PASADENA provides a low-cost alternative for high throughput.This equipment is suited to investigators who have an established small animal NMR and wish to explore the potential of heteronuclear ((13) C and (15) N) MRI, MRS, which harnesses the enormous sensitivity gain offered by hyperpolarization.