INTRACELLULAR STUDY OF DENDRODENDRITIC INHIBITORY SYNAPSES ON MITRAL CELLS IN RABBIT OLFACTORY-BULB
INTRACELLULAR STUDY OF DENDRODENDRITIC INHIBITORY SYNAPSES ON MITRAL CELLS IN RABBIT OLFACTORY-BULB
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DOI：
10.1113/jphysiol.1978.sp012362
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发表时间：
1978-01-01
影响因子：
5.5
通讯作者：
TAKAGI, SF
中科院分区：
文献类型：
--
作者：
MORI, K;TAKAGI, SF
在兔嗅球上，记录到刺激侧嗅束(Lot)后二尖瓣细胞和颗粒细胞层(GCL)神经元的细胞内电位。多数来自二尖瓣细胞的记录显示反向峰后IPSP[抑制性突触后电位]较大(5-21 mV)和延长(60-650ms)。这些IPSP的幅度降低，然后通过细胞内施加的超极化电流的进行性增加而反转。伴随而来的是二尖瓣细胞膜电导显著而持久的增加(高达100ms)。二尖瓣细胞反向IPSP与原来的超极化IPSP有很大的不同，提示抑制性突触广泛分布于胞体和树突上。GCL细胞可记录到E[兴奋性]PSP，其起始潜伏期比二尖瓣细胞IPSP短约0.6ms。比较GCL细胞和二尖瓣细胞IPSP在不同LOT刺激条件下的行为，提示这些GCL细胞是介导二尖瓣细胞抑制的抑制性中间神经元。显然，存在激活颗粒细胞和随后抑制二尖瓣细胞的树突状通路。
In the rabbit olfactory bulb, intracellular potentials were recorded from mitral cells and from neurons in the granule cell layer (GCL) following lateral olfactory tract (LOT) stimulation. Most recordings from mitral cells showed large (5-21 mV) and prolonged (60-650 ms) IPSP [inhibitory postsynaptic potential] subsequent to the antidromic spikes. These IPSP decreased in amplitude and then reversed in polarity by progressive increase in hyperpolarizing current applied intracellularly. They were accompanied by a prominent and long lasting (up to 100 ms) conductance increase of the mitral cell membrane. Reversed IPSP of mitral cells having quite different time courses from the original hyperpolarizing IPSP suggested that the inhibitory synapses are widely distributed on the soma and dendrites. E[excitatory]PSP could be recorded from GCL cells whose onset latency was approximately 0.6 ms shorter than that of mitral cell IPSP. Comparison of the behavior of EPSP in GCL cells and that of mitral cell IPSP under various conditions of LOT stimulation suggested that these GCL cells are the inhibitory interneurons mediating mitral cell inhibition. Apparently there are dendrodendritic pathways for activation of granule cells and subsequent inhibition of mitral cells.