Collision Cross-Section Measurements of Collision-Induced Dissociation Precursor and Product Ions in an FTICR-MS and an IM-MS: A Comparative Study
Collision Cross-Section Measurements of Collision-Induced Dissociation Precursor and Product Ions in an FTICR-MS and an IM-MS: A Comparative Study
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FTICR-MS 和 IM-MS 中碰撞诱导解离母离子和子离子的碰撞截面测量：比较研究
DOI：
10.1021/jasms.2c00089
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发表时间：
2022
影响因子：
3.2
通讯作者：
Dearden, David V.
中科院分区：
文献类型：
--
作者：
Arslanian, Andrew J.;Mismash, Noah;Dearden, David V.
傅里叶变换离子回旋共振质谱法（SORI-CRAFTI）的持续非共振辐射截面积是一种FTICR-MS策略，用于碰撞激活前体离子，然后同时测量其离子-中性碰撞截面以及所选产物的离子-中性碰撞截面。我们使用质子化亮氨酸脑啡肽对SORI-CRAFTI进行基准测试，与先前通过碰撞诱导解离离子迁移率（CID-IMS）进行的研究非常一致（通常在1-2%范围内）。然后将SORI-CRAFTI应用于碱金属阳离子化亮氨酸脑啡肽，并通过前体/产物横截面比与CID-IMS进行比较。SORI-CRAFTI和CID-IMS之间的定性一致性非常好（同样，通常在1-2%内）;然而，SORI-CRAFTI和CID-IMS都不能确定金属化亮氨酸脑啡肽是否以其典型或两性离子形式存在。当SORI-CRAFTI用于[2.2.2]-穴状配体+Cs+时，SORI活化导致碰撞截面减少5%，与外部结合的Cs+迁移到穴状配体的腔中一致，并且与从富含异丙醇的溶剂电喷雾时观察到的截面相似。因此，SORI-CRAFTI可用于研究小到中等大小的分子和主客体系统的气相离子化学。
Sustained off-resonance irradiation-cross-sectional areas by Fourier transform ion cyclotron resonance mass spectrometry (SORI-CRAFTI) is an FTICR-MS strategy to collisionally activate precursor ions and then measure their ion-neutral collision cross sections, as well as those of selected products, at the same time. We benchmarked SORI-CRAFTI using protonated leucine-enkephalin, to excellent agreement (typically within 1–2%) with previous studies performed via collision-induced dissociation-ion mobility (CID-IMS). SORI-CRAFTI was then applied to alkali metal-cationized leucine-enkephalin and compared with CID-IMS via precursor/product cross-section ratios. Qualitative agreement between SORI-CRAFTI and CID-IMS was excellent (again, usually within 1–2%); however, neither SORI-CRAFTI nor CID-IMS could determine if metalated leucine-enkephalin was present in its canonical or zwitterionic form. When SORI-CRAFTI was used on [2.2.2]-cryptand+Cs+, SORI activation resulted in a 5% decrease in collision cross section, consistent with migration of the externally bound Cs+into the cryptand’s cavity and similar to the cross section observed when electrospraying from an isopropanol-rich solvent. Thus, SORI-CRAFTI is useful for studying gas-phase ion chemistry of small- to medium-sized molecules and host–guest systems.