Oxalic acid and sclerotial differentiation of Polyporus umbellatus.
Oxalic acid and sclerotial differentiation of Polyporus umbellatus.
复制标题
草酸与猪苓菌核分化
DOI：
10.1038/srep10759
复制
发表时间：
2015-06-01
影响因子：
4.6
通讯作者：
Guo SX
中科院分区：
文献类型：
--
作者：
Xing YM;Yin WQ;Liu MM;Wang CL;Guo SX
本研究旨在揭示外源草酸对猪苓菌核形成过程的影响，重点是测定真菌中内源草酸的含量。为此，使用高效液相色谱法（HPLC）测量营养菌丝体、菌核、培养基和菌核渗出物的草酸含量。此外，通过检测硫代巴比妥酸反应物质（TBARS）来估计脂质过氧化。结果表明，外源草酸导致菌核分化延迟（长达9天或更长时间），抑制菌核生物量，并以浓度依赖性方式显着降低脂质过氧化。在菌丝体和麦芽糖培养基中发现草酸含量非常低，而在菌丝体和蔗糖培养基中发现草酸含量很高。菌核分化后，草酸在菌核和菌核渗出液中积累高水平。因此发现草酸可以抑制伞形孢菌菌核的形成。
The present investigation aimed to uncover the effects of exogenous oxalic acid during the sclerotial formation of Polyporus umbellatus, with an emphasis on determining the content of the endogenic oxalic acid in the fungus. To this end, the oxalic acid content of the vegetative mycelia, sclerotia, culture mediums and sclerotial exudate were measured using High Performance Liquid Chromatography (HPLC). Furthermore, the lipid peroxidation was estimated by detecting thiobarbituric bituric acid reactive substances (TBARS). The results showed that the exogenous oxalic acid caused a delay in sclerotial differentiation (of up to 9 or more days), suppressed the sclerotial biomass and decreased the lipid peroxidation significantly in a concentration-dependent manner. Oxalic acid was found at very low levels in the mycelia and the maltose medium, whereas it was found at high levels in the mycelia and sucrose medium. After sclerotial differentiation, oxalic acid accumulated at high levels in both the sclerotia and the sclerotial exudate. Oxalic acid was therefore found to inhibit P. umbellatus sclerotial formation.