Bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from Japanese subjects
Bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from Japanese subjects
复制标题
DOI：
10.1016/j.archoralbio.2009.01.010
复制
发表时间：
2009-04-01
影响因子：
3
通讯作者：
Ooshima, Takashi
中科院分区：
文献类型：
--
作者：
Miyamoto, Eriko;Nakano, Kazuhiko;Ooshima, Takashi
目的：分子生物学技术的最新发展增加了对临床标本中口腔细菌种类分布的了解，尽管在同一标本中同时检测口腔链球菌和牙周细菌种类的调查很少。本研究旨在探讨儿童及其母亲唾液标本中6种口腔链球菌和6种牙周链球菌的分布及其相关性。设计：74对儿童及其母亲被批准参加本研究。收集唾液标本，提取细菌DNA，使用物种特异性引物进行PCR分析。通过统计分析确定能够同时检测到的物种组合。结果：母亲中链球菌和牙龈卟啉单胞菌的检出率高于儿童。变形链球菌，血链球菌和链球菌；在大量标本中同时检测到口腔弯曲杆菌，而直弯曲杆菌的存在与至少一种红色复合体（牙龈假单胞菌、齿龈密螺旋体和连生单宁菌）的存在相关。另一方面，口腔链球菌的检出率与牙周菌种之间没有相关性。结论：我们的研究结果表明，在链球菌和牙周菌种中，有几种可以在唾液中共存，而两者的存在并不相互影响。2009爱思唯尔有限公司版权所有。
Objective: Recent developments in molecular biological techniques have increased understanding of the distribution of oral bacterial species in clinical specimens, though few investigations have been conducted to simultaneously detect oral streptococcal and periodontal species in the same specimens. The purpose of the present study was to investigate the distribution and correlation of 6 oral streptococcal and 6 periodontal species in saliva specimens taken from children and their mothers.Design: Seventy-four pairs of children and their mothers were approved to participate in this study. Saliva specimens were collected and bacterial DNA extracted, which was subjected to PCR analyses using species-specific sets of primers. The combinations of species able to be detected simultaneously were determined by statistical analyses.Results: Streptococcus sobrinus and Porphyromonas gingivalis were detected more often in the mothers than the children. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus; oralis were detected simultaneously in a significant number of specimens, while the presence of Campylobacter rectus was correlated with the presence of at least one of the red complex species (P. gingivalis, Treponema denticola and Tannerella forsythensis). On the other hand, no correlation was shown between the rates of detection of oral streptococcal and periodontal species.Conclusions: Our results indicate that among streptococcal and periodontal species, several are able to coexist in saliva, while the presence of both does not have an influence on each other. (C) 2009 Elsevier Ltd. All rights reserved.