Lung injury following lower extremity blast trauma in rats
Lung injury following lower extremity blast trauma in rats
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大鼠下肢爆炸伤后的肺损伤
DOI：
10.1097/ta.0b013e318266013a
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发表时间：
2012-12-01
影响因子：
3.4
通讯作者：
Ma, Daqing
中科院分区：
文献类型：
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作者：
Ning, Jiao-Lin;Mo, Li-Wen;Ma, Daqing
背景下肢爆炸伤是武装冲突和恐怖袭击后常见的创伤，死亡率高，可能与远处重要器官损伤有关。本研究旨在探讨爆炸性下肢创伤后远端肺损伤的机制。方法将SD大鼠随机分为假手术组（Sham）和爆炸组（Blast）。爆炸组使用图表状电雷管对左后肢进行爆炸创伤，然后将其细分为它们被处死的时间：0.5，1，3和6小时。假手术组又分为基线对照组和时程组。基线组在动脉插管后0.5小时处死，假爆炸后6小时处死。收获肺用于组织学分析和含水量测量。在每个实验结束时收集血液样品，并分析细胞因子、髓过氧化物酶、丙二醛、超氧化物歧化酶和胱硫醚裂解酶活性和硫化氢。结果爆炸伤后大鼠肺组织含水量增加，肺泡细胞浸润和肺泡损伤呈时间依赖性。血浆和肺的促炎细胞因子，肿瘤坏死因子-和白细胞介素6，和丙二醛的水平，被认为是显着增加，连同髓过氧化物酶的上升和超氧化物歧化酶，胱硫醚裂解酶，硫化氢的同时下降。结论肢体爆炸伤可引起远隔肺损伤，这可能与显著的炎症反应、氧化应激和保护机制缺失有关。
BACKGROUND Lower extremity blast trauma is a common injury during armed conflict and after terrorist attacks with a high mortality, which is likely associated with distant vital organ injury. The current study aimed to investigate the underlying mechanisms of remote lung injury after blast lower extremity trauma. METHODS Sprague-Dawley rats were randomly divided into two groups: sham and blast. The blast group underwent blast trauma to the left hind limb using chartaceous electricity detonators, which was then subdivided into the time at which they were sacrificed: 0.5, 1, 3, and 6 hours. The sham group was also subdivided into the baseline control and time course groups. The baseline group was sacrificed 0.5 hours after artery cannulation and the time course at 6 hours after sham blast. The lungs were harvested for histologic analysis and water content measurement. Blood samples were harvested at each end of experiment and analyzed for cytokines, myeloperoxidase, malondialdehyde, and superoxide dismutase and cystathionine &ggr;-lyase activity and hydrogen sulfide. RESULTS Blast hind limb trauma induced alveolar injury and cell infiltration, together with an increase in lung water content, in a time-dependent manner. Plasma and lung levels of proinflammatory cytokines, tumor necrosis factor-&agr; and interleukin 6, and malondialdehyde, were found to be significantly increased in conjunction with a rise in myeloperoxidase and a concurrent fall in superoxide dismutase, cystathionine &ggr;-lyase, and hydrogen sulfide. CONCLUSION Our data demonstrated that blast limb trauma causes remote lung injury, which is likely associated with remarkable inflammatory response, oxidative stress, and depletion of protective mechanisms.