[Modified Heidelberg Retinal Tomograph HRT. Initial results of in vivo presentation of corneal structures].
[Modified Heidelberg Retinal Tomograph HRT. Initial results of in vivo presentation of corneal structures].
复制标题
[改良海德堡视网膜断层扫描 HRT。
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
R. Guthoff
中科院分区：
文献类型：
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作者：
J. Stave;G. Zinser;G. Grümmer;R. Guthoff
背景
目前，使用带卤素或汞灯的共聚焦串联扫描显微镜来描绘活体所有的角膜结构，例如PRK或LASIK前后的结构。在应用该仪器时，成像质量不足和角膜光照不规则是自动定量评估角膜细胞密度的主要问题。校正图片的背景不规则性需要很高的校正量。我们的目的是了解是否有可能将海德堡视网膜断层扫描仪(HRT)改造成高分辨率数字激光扫描显微镜，用于眼前节的可视化，并配以特殊的评估软件。
材料和方法
我们开发了一种用于HRT的透镜适配器，通过与外部的计算机控制的液压z扫描雪橇相结合，将激光聚焦到角膜上。通过结合所有内部控制功能和患者数据，使用Z-SCAN雪橇上外部步进电机的可编程适配，可以创建保留所有原始HRT功能的数字共焦激光扫描显微镜。为了评价角膜图像和角膜细胞的自动计数，我们使用了特殊的3D和Chemoaxis软件。
结果
最初的研究表明，所有的角膜结构，如角膜上皮、内皮和角质形成细胞，都有规律的光照。液压z扫描可以精确地将焦点移过角膜，拍摄一系列图像，以评估角膜细胞的轮廓和所有角膜结构的3D重建。
BACKGROUND
At present, confocal tandem scanning microscopes with halogen or mercury lamps are used to depict all corneal structures in vivo, e.g., before and after PRK or LASIK. Insufficient imaging quality and irregular corneal illumination are the main problems for automatic quantitative evaluation of the keratocyte density when applying this instrument. A high correction is required for correcting the background irregularities of pictures. Our aim was to find out whether it is possible to change the Heidelberg retina tomograph (HRT) into a high-resolution digital laser scanning microscope for the visualization of anterior segments of the eye, coupled with a special evaluation software.
MATERIAL AND METHOD
We developed a lens adapter for the HRT that focusses the laser beam onto the cornea by combining with an external, computer-controlled hydraulic z-scan sledge. By using a programmable adaptation for the external stepmotor on the z-scan sledge in combination with all internal control functions and patient data, it is possible to create a digital confocal laser scanning microscope with retention of all the original HRT functions. For evaluation of the corneal images and automatic count of keratocytes, we used special 3D and Chemotaxis software.
RESULTS
First investigations show a regular illumination of all corneal structures as the epithelium, endothelium, and keratocytes. The hydraulic z-scan allowed a precise shift of the focus through the cornea to take series of images for the evaluation of the keratocyte profile and 3D reconstruction of all corneal structures.