On-line coupling of microdialysis sampling with microchip-based capillary electrophoresis
On-line coupling of microdialysis sampling with microchip-based capillary electrophoresis
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DOI：
10.1021/ac049365i
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发表时间：
2004-11-01
影响因子：
7.4
通讯作者：
Lunte, SM
中科院分区：
文献类型：
--
作者：
Huynh, BH;Fogarty, BA;Lunte, SM
微透析取样是一种已用于体内和体外监测药物，生物医学和环境利益的化合物的技术。介绍了一种市售微透析探针与基于微芯片的毛细管电泳（CE）系统的耦合。将来自微透析探针的连续流动的透析液流引入微芯片中，并使用无阀门控方法实现离散注射。研究了施加电压和微透析流速对装置性能的影响。结果表明，峰面积与外加电压呈线性关系。较高的电压导致较低的峰响应，但更快的分离。发现0.8和1.0 μ L/min的灌注流速可提供最佳性能。采用在线微透析/微芯片毛细管电泳系统对β-D-半乳糖苷酶水解荧光素单-β-D-吡喃半乳糖苷（FMG）的过程进行了监测。观察到FMG底物减少，荧光素产物增加。设备的时间分辨率（取决于CE分离时间）为30 s。据我们所知，这是第一次报道耦合的微透析采样探针的微芯片毛细管电泳装置。
Microdialysis sampling is a technique that has been used for in vivo and in vitro monitoring of compounds of pharmaceutical, biomedical, and environmental interest. The coupling of a commercially available microdialysis probe to a microchip-based capillary electrophoresis (CE) system is described. A continuously flowing dialysate stream from a microdialysis probe was introduced into the microchip, and discrete injections were achieved using a valveless gating approach. The effect of the applied voltage and microdialysis flow rate on device performance was investigated. It was found that the peak area varied linearly with the applied voltage. Higher voltages led to lower peak response but faster separations. Perfusion flow rates of 0.8 and 1.0muL/min were found to provide optimal performance. The on-line microdialysis/microchip CE system was used to monitor the hydrolysis of fluorescein mono-beta-D-galactopyranoside (FMG) by beta-D-galactosidase. A decrease of the FMG substrate with an increase in the fluorescein product was observed. The temporal resolution of the device, which is dependent on the CE separation time, was 30s. To the best of our knowledge, this is the first reported coupling of a microdialysis sampling probe to a microchip capillary electrophoresis device.