ABSOLUTE QUANTIFICATION OF DEOXYHEMOGLOBIN CONCENTRATION IN TISSUE NEAR-INFRARED SPECTROSCOPY
ABSOLUTE QUANTIFICATION OF DEOXYHEMOGLOBIN CONCENTRATION IN TISSUE NEAR-INFRARED SPECTROSCOPY
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DOI：
10.1088/0031-9155/39/8/008
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发表时间：
1994-08-01
影响因子：
3.5
通讯作者：
COOPER, CE
中科院分区：
文献类型：
--
作者：
MATCHER, SJ;COOPER, CE
我们描述了一种简单的技术，用于非侵入性地确定脱氧血红蛋白（Hb）的绝对浓度在活组织中使用近红外光谱（NIRS）。该技术使用二阶微分光谱法，通过拟合这两种发色团在近红外（NIR）光谱的710 nm至840 nm区域的光谱特征来确定Hb与组织水的相对浓度。由于组织水的浓度通常以百分之几的精度已知，因此可以获得Hb的绝对浓度（[Hb]）。该技术的有效性和可能的准确性进行评估，将其应用于人工生成的近红外光谱数据。通过比较，在体内研究中，通过这种方法获得的[Hb]的变化和使用更传统的相对定量技术计算的变化，提出了一些临床验证。最后，我们讨论了绝对血红蛋白浓度的测量可能的临床意义。
We describe a simple technique for non-invasively determining the absolute concentration of deoxyhaemoglobin (Hb) in living tissue using near infrared spectroscopy (NIRS). The technique uses second-differential spectroscopy to determine the relative concentration of Hb to tissue water by fitting of the spectral features of these two chromophores in the 710 nm to 840 nm region of the near infrared (NIR) spectrum. Since the concentration of tissue water is generally known with accuracies of a few per cent, one can then obtain the absolute concentration of Hb ([Hb]). The validity and likely accuracy of the technique is assessed by applying it to artificially generated NIRs data. Some clinical validation is presented by comparing, during an in vivo study, changes in [Hb] obtained by this method and those calculated using more conventional techniques of relative quantification. Finally, we discuss the likely clinical significance of the measurement of absolute Hb concentration.