Distinctive functional properties of human blood L lymphocytes: a comparison with T lymphocytes, B lymphocytes, and monocytes.
Distinctive functional properties of human blood L lymphocytes: a comparison with T lymphocytes, B lymphocytes, and monocytes.
复制标题
人血 L 淋巴细胞的独特功能特性：与 T 淋巴细胞、B 淋巴细胞和单核细胞的比较。
DOI：
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发表时间：
1977
影响因子：
4.4
通讯作者：
Martha Anne Garrett
中科院分区：
文献类型：
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作者：
D. Horwitz;Martha Anne Garrett
具有高亲和力Fc受体的人血淋巴细胞因具有膜不稳定的免疫球蛋白G标记而被命名为L淋巴细胞。L淋巴细胞缺乏膜结合免疫球蛋白，不能与包被免疫球蛋白抗体包被的绵羊红细胞和小鼠补体形成花环。这些淋巴细胞能在4℃下与正常人血清中的免疫球蛋白结合，并与包被Ripley免疫球蛋白的人淋巴细胞形成花环。本研究比较了分离的L淋巴细胞与T淋巴细胞、B淋巴细胞和单核细胞的体外功能特性。为了获得这些单核细胞群，首先，收集塑料贴壁的单核细胞。用E玫瑰花环形成细胞离心法分离T淋巴细胞，用其他玫瑰花环技术通过负选择分离L和B淋巴细胞。L瘤细胞的功能特性完全不同于T细胞、B细胞或单核细胞。L淋巴细胞对有丝分裂原、可溶性抗原和细胞表面抗原均无增殖反应。此外，这一群体不能取代单核细胞来帮助T淋巴细胞对刀豆蛋白A和商陆有丝分裂原做出反应。然而，在T细胞中加入单核细胞后，加入L淋巴细胞可显著增强T淋巴细胞对植物血凝素、刀豆蛋白A、纯化蛋白衍生物和链激酶/链多糖酶的增殖反应。L淋巴细胞不属于B细胞亚群。它们在培养中不会自发产生表面免疫球蛋白，PokWeek有丝分裂原不能诱导它们转化并产生免疫荧光检测到的细胞质免疫球蛋白。混合的B细胞和T细胞对商陆有丝分裂原的反应比单独使用T细胞更好。相反，没有观察到与L和T细胞组合的反应性增强。这两个群体之间的另一个显著差异是在混合淋巴细胞培养中各自的刺激能力。当仔细去除B和L淋巴细胞的单核细胞时，只有B细胞是自体和同种异体淋巴细胞的有效刺激因子。与T细胞、B细胞和单核细胞相比，L淋巴细胞是免疫球蛋白致敏的人血淋巴细胞的唯一有效杀伤细胞。因此，L淋巴细胞具有细胞毒潜力，但不能对各种刺激剂做出反应而增殖，也不能成为产生抗体的细胞。这些发现表明，L淋巴细胞构成了第三淋巴细胞群。
Human blood lymphocytes with high affinity Fc receptors have been operationally named L lymphocytes because of membrane-labile IgG markers. L lymphocytes lack membrane-incorporated immunoglobulin and do not form rosettes with sheep red blood cells coated with IgM antibody and mouse complement. These lymphocytes are capable of binding IgG in normal human serum at 4 degrees C and will form rosettes with human lymphocytes coated with Ripley IgG. In this study, functional in vitro properties of isolated L lymphocytes were compared with T lymphocytes, B lymphocytes, and monocytes. To obtain these mononuclear populations, first, plastic adherent monocytes were harvested. T lymphocytes were then isolated by centrifugation of E rosette-forming cells, and other rosetting techniques were employed to isolate L and B lymphocytes by negative selection. The functional properties of L lumphocytes were completely unlike those of T cells, B cells, or monocytes. L lymphocytes did not proliferate in response to mitogens, soluble antigens, or cell surface antigens. Moreover, this population could not replace monocytes in helping T lymphocytes respond to concanavalin A and pokeweed mitogen. Once T cells were supplemented with monocytes, however, the addition of L lymphocytes to the culture greatly enhanced the T lymphocytes proliferative response to phytohemagglutinin, concanavalinA, purified protein derivative (PPD), and streptokinase/streptodornase. L lymphocytes were not a subset of B cells. They did not spontaneously develop surface Ig in culture, and pokeweek mitogen could not induce them to transform and generate cytoplasmic Ig detectable by immunofluorescence. Mixtures of B cells and T cells responded to pokeweed mitogen better than do T cells alone. In contrast, enhanced reactivity with L and T cell combinations was not observed. Another sharp difference between these two populations was the stimulator capacity of each in mixed lymphocyte culture. When B and L lymphocytes were carefully monocyte-depleted, only B cells were effective stimulators of autologous and allogeneic lymphocytes. In comparison with T cells, B cells, and monocytes, L lymphocytes were the only effective killers of human blood lymphocytes sensitized with IgG. L lymphocytes, then, have cytotoxic potential, but cannot proliferate in response to various stimulants or become antibody-producing cells. These findings suggest that L lymphocytes comprise a third lymphocyte population.