The Hybrid Optical and Photoacoustic Microscopy: a Novel System to Image Morphological and Photoacoustic Characteristics of Cells
The Hybrid Optical and Photoacoustic Microscopy: a Novel System to Image Morphological and Photoacoustic Characteristics of Cells
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DOI：
10.1109/ius46767.2020.9251548
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发表时间：
2020-09
期刊：
影响因子：
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通讯作者：
Ryo Shintate;Ryo Nagaoka;T. Ishii;Y. Saijo
中科院分区：
文献类型：
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作者：
Ryo Shintate;Ryo Nagaoka;T. Ishii;Y. Saijo
光学分辨率光声显微镜 (OR-PAM) 可以实现光学依赖性的高横向分辨率 (<1 µm)，并在单细胞水平上可视化光声 (PA) 特性。通过将获得的 PA 特征与细胞形态进行比较，PA 成像的空间分辨率有望精确表征细胞的生物活性，例如细胞与药物和造影剂的相互作用。然而，由于传统的OR-PAM系统仅可视化产生PA信号的物体，因此很难同时获取PA图像和光学形态图像。在这里，我们报告了一种新型混合光学/PA显微镜系统，可以同时获取细胞的形态和PA特征。通过测量 USAF1951 目标和牛红细胞来验证所开发系统的性能。
Optical resolution photoacoustic microscopy (OR-PAM) can achieve an optical-dependent high lateral resolution (<1 µm) and visualize photoacoustic (PA) property at single-cell level. The spatial resolvability of the PA imaging is expected to precisely characterize biological activities of cells such as their interactions with drugs and contrast agents by comparing the acquired PA characteristics with the cell's morphology. However, since the conventional OR-PAM systems only visualize objects that generate PA signals, it was difficult to acquire a PA image and an optical morphological image simultaneously. Here, we report a novel system of the hybrid optical / PA microscopy that can acquire morphological and PA characteristics of cells at the same time. The performance of the developed system was validated by measuring a USAF1951 target and bovine red blood cells.