3,400 NEW EXPRESSED SEQUENCE TAGS IDENTIFY DIVERSITY OF TRANSCRIPTS IN HUMAN BRAIN
3,400 NEW EXPRESSED SEQUENCE TAGS IDENTIFY DIVERSITY OF TRANSCRIPTS IN HUMAN BRAIN
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DOI：
10.1038/ng0793-256
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发表时间：
1993-07-01
期刊：
影响因子：
30.8
通讯作者：
VENTER, JC
中科院分区：
文献类型：
--
作者：
ADAMS, MD;KERLAVAGE, AR;VENTER, JC
我们展示了来自人脑cDNA克隆的超过3400个表达序列标签（est）的部分测序结果，这将以est为代表的大脑中表达的不同基因的数量增加到大约6000个。通过以公正的方式选择克隆，可以构建不同阶段大脑转录活动的概况。构成细胞骨架的蛋白质是最丰富的；然而，也发现了大量的调节蛋白。预计含有蛋白质编码区域的est中约有一半在公共肽数据库中没有匹配，可能代表新的基因家族。
We present the results of the partial sequencing of over 3,400 expressed sequence tags (ESTs) from human brain cDNA clones, which increases the number of distinct genes expressed in the brain, that are represented by ESTs, to about 6,000. By choosing clones in an unbiased manner, it is possible to construct a profile of the transcriptional activity of the brain at different stages. Proteins that comprise the cytoskeleton are the most abundant; however, a large variety of regulatory proteins are also seen. About half of the ESTs predicted to contain a protein-coding region have no matches in the public peptide databases and may represent new gene families.