Relevance of nuclear localization and functions of RNA helicase A.
Relevance of nuclear localization and functions of RNA helicase A.
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DOI：
10.3892/ijmm.15.4.555
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发表时间：
2005-04
影响因子：
5.4
通讯作者：
Hidetoshi Fujita;T. Ohshima;T. Oishi;S. Aratani;R. Fujii;A. Fukamizu;T. Nakajima
中科院分区：
文献类型：
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作者：
Hidetoshi Fujita;T. Ohshima;T. Oishi;S. Aratani;R. Fujii;A. Fukamizu;T. Nakajima
RNA解旋酶A （RHA）在转录和转录后水平调控基因表达。我们还知道RHA在细胞核和细胞质之间穿梭。RHA具有核输运域，作为核定位信号（NLS）和核输出信号（NES），主要定位于细胞核内。在这里，我们确定了影响RHA细胞定位的区域。我们发现双链RNA结合域I和II （dsRBD I和II）正定位于细胞质。突变分析表明，rna结合能力对dsRBD在细胞质中的定位很重要。缺乏与Pol II复合物结合的RHA突变体部分从细胞核外溢。此外，与GFP相比，最小的反活化结构域（MTAD）主要定位于细胞核，这是与Pol II复合物相互作用的最小区域。结果表明，RHA的细胞内定位可能不仅受NLS和NES的调控，还受功能域的调控。
RNA helicase A (RHA) regulates gene expression at transcriptional and post-transcriptional levels. It is also known that RHA shuttles between the nucleus and the cytoplasm. RHA possesses nuclear transport domain which functions as a nuclear localization signal (NLS) and nuclear export signal (NES), and predominantly localizes in the nucleus. Here, we identified regions that influence cellular localization of RHA. We showed that double-stranded RNA binding domain I and II (dsRBD I and II) positively localized to the cytoplasm. Mutational analysis revealed that RNA-binding ability was important to localization of dsRBD to the cytoplasm. RHA mutants lacking association with Pol II complexes were partially spilled from the nucleus. Furthermore, a minimal transactivation domain (MTAD), which is minimal region to interact with Pol II complexes, mainly localized to the nucleus in comparison with GFP. Our results indicated that the intracellular localization of RHA might be regulated by not only NLS and NES but also functional domains.