Self-assembled monolayers that resist the adsorption of proteins and the adhesion of bacterial and mammalian cells
Self-assembled monolayers that resist the adsorption of proteins and the adhesion of bacterial and mammalian cells
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DOI：
10.1021/la010552a
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发表时间：
2001-10-02
期刊：
影响因子：
3.9
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Ostuni, E;Chapman, RG;Whitesides, GM
本文检验了这样的假设：抗蛋白质吸附的表面也应能抵抗细菌（金黄色葡萄球菌、表皮葡萄球菌）的粘附以及哺乳动物细胞（牛毛细血管内皮 (BCE) 细胞）的附着和扩散。测试的表面是自组装单分子层的表面； (SAM)以三(肌氨酸)(Sarc)、N-乙酰哌嗪、全甲基化山梨醇、六甲基磷酰胺、磷酰胆碱和分子内两性离子(-CH2N+(CH3)(2)CH2CH2CH2SO3-)(ZW)的衍生物封端；已知所有这些都可以抵抗蛋白质的吸附。蛋白质（纤维蛋白原和溶菌酶）的吸附与细胞的粘附之间似乎几乎没有或没有相关性。用 Sarc 和 N-乙酰哌嗪衍生物封端的表面与用三乙二醇封端的表面一样，抵抗金黄色葡萄球菌和表皮葡萄球菌的粘附。呈现 Sarc 基团的表面是唯一能够抵抗 BCE 细胞粘附的表面，并且表面以三（乙二醇）封端。 BCE细胞与表面的附着可以使用以Sarc、N-乙酰哌嗪、磷酰胺和ZW的衍生物封端的SAM作为抗附着成分以及以甲基封端的SAM作为粘合成分来图案化。
This paper examines the hypothesis that surfaces resistant to protein adsorption should also be resistant to the adhesion of bacteria (Staphylococcus aureus, Staphylococcus epidermidis) and the attachment and spreading of mammalian cells (bovine capillary endothelial (BCE) cells). The surfaces tested were those of self-assembled monolayers; (SAMs) terminated with derivatives of tri(sarcosine) (Sarc),N-acetylpiperazine, permethylated sorbitol, hexamethylphosphoramide, phosphoryl choline, and an intramolecular zwitterion (-CH2N+(CH3)(2)CH2CH2CH2SO3-) (ZW); all are known to resist the adsorption of proteins. There seems to be little or no correlation between the adsorption of protein (fibrinogen and lysozyme) and the adhesion of cells. Surfaces terminated with derivatives of Sarc and N-acetylpiperazine resisted the adhesion of S. aureus and S. epidermidis as well as did surfaces terminated with tri(ethylene glycol). A surface that presented Sarc groups was the only one that resisted the adhesion of BCE cells as well as did a surface terminated with tri(ethylene glycol). The attachment of BCE cells to surfaces could be patterned using SAMs terminated with derivatives of Sarc, N-acetylpiperazine, phosphoramide, and the ZW as the attachment-resistant component and methyl-terminated SAMs as the adhesive component.