Quantifying Immune Cell Force Generation Using Traction Force Microscopy.
Quantifying Immune Cell Force Generation Using Traction Force Microscopy.
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DOI：
10.1007/978-1-0716-3135-5_23
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发表时间：
2023-01-01
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影响因子：
--
通讯作者：
Fritzsche, Marco
中科院分区：
文献类型：
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作者：
Issler, Marcel;Colin-York, Huw;Fritzsche, Marco
免疫细胞依靠机械力的产生来执行它们的功能。因此，迫切需要能够探测免疫细胞产生的机械力的时空分布的定量方法。在本章中，我们提供了使用牵引力显微镜(TFM)量化免疫细胞力量生成的指南，并特别关注其在T细胞免疫突触研究中的应用。
Immune cells rely on the generation of mechanical force to carry out their function. Consequently, there is a pressing need for quantitative methodologies that permit the probing of the spatio-temporal distribution of mechanical forces generated by immune cells. In this chapter, we provide a guide to quantify immune cell force generation using traction force microscopy (TFM), with a specific focus on its application to the study of the T-cell immunological synapse.