Evolution at two levels of gene expression in yeast.
Evolution at two levels of gene expression in yeast.
复制标题
DOI：
10.1101/gr.165522.113
复制
发表时间：
2014-03
期刊：
影响因子：
7
通讯作者：
Fraser HB
中科院分区：
文献类型：
--
作者：
Artieri CG;Fraser HB
尽管蛋白质水平的功能更重要，但我们对基因表达进化的认识几乎完全基于mRNA水平的研究。相比之下，我们对翻译调控如何进化的理解远远落后。在这里，我们已经应用核糖体分析，这两个全球mRNA水平和翻译率的措施，以两个物种的酵母菌及其种间杂交，以评估mRNA丰度和翻译的变化对调控进化的相对贡献。我们报告说，无论是顺式和反式作用的监管分歧翻译是丰富的，影响至少35%的基因。大多数翻译分歧的作用，以缓冲mRNA丰度的变化，这表明了一个广泛的作用，稳定的选择跨监管水平。尽管如此，我们观察到的证据，谱系特异性选择作用于几个酵母功能模块，包括加强选择的情况下，在两个层次的监管。最后，我们还发现了物种之间保守的终止密码子通读的多个实例。我们的分析揭示了未被充分认识的转录后调控分歧的复杂性，并表明将选择位点的搜索划分为“编码”与“调控”的二元类别可能会忽略一个重要的选择来源，它沿着从基因型到表型的路径在多个调控水平上起作用。
Despite the greater functional importance of protein levels, our knowledge of gene expression evolution is based almost entirely on studies of mRNA levels. In contrast, our understanding of how translational regulation evolves has lagged far behind. Here we have applied ribosome profiling—which measures both global mRNA levels and their translation rates—to two species of Saccharomyces yeast and their interspecific hybrid in order to assess the relative contributions of changes in mRNA abundance and translation to regulatory evolution. We report that both cis- and trans-acting regulatory divergence in translation are abundant, affecting at least 35% of genes. The majority of translational divergence acts to buffer changes in mRNA abundance, suggesting a widespread role for stabilizing selection acting across regulatory levels. Nevertheless, we observe evidence of lineage-specific selection acting on several yeast functional modules, including instances of reinforcing selection acting at both levels of regulation. Finally, we also uncover multiple instances of stop-codon readthrough that are conserved between species. Our analysis reveals the underappreciated complexity of post-transcriptional regulatory divergence and indicates that partitioning the search for the locus of selection into the binary categories of “coding” versus “regulatory” may overlook a significant source of selection, acting at multiple regulatory levels along the path from genotype to phenotype.
登录
查看更多内容
影响因子：
9.8
作者：
Charneski CA;Hurst LD
通讯作者：
Hurst LD
影响因子：
9.9
作者：
Bentele, Kajetan;Saffert, Paul;Bluethgen, Nils
通讯作者：
Bluethgen, Nils
两个层面的进化：基因和形态。
影响因子：
9.8
作者：
Carroll, SB
通讯作者：
Carroll, SB
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1073/pnas.0812009106
发表时间：
2009-01-27
影响因子：
11.1
作者：
Bedford, Trevor;Hartl, Daniel L.
通讯作者：
Hartl, Daniel L.