Collagen antibody-induced arthritis
Collagen antibody-induced arthritis
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DOI：
10.1038/nprot.2006.393
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发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Khachigian, Levon M.
中科院分区：
文献类型：
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作者：
Khachigian, Levon M.
胶原抗体诱导的关节炎（CAIA）是一种简单的类风湿性关节炎小鼠模型，可用于解决致病机制问题并筛选候选治疗药物。关节炎是通过施用针对II型胶原蛋白中保守的自身抗原表位的单克隆抗体的混合物，然后施用内毒素来刺激的。抗体诱导的关节炎模型提供了几个关键的优势，超过经典的胶原蛋白诱导的关节炎（CIA）模型。这些包括快速发病，高摄取率，同步性，以及使用转基因小鼠，如转基因和基因敲除小鼠的能力。该方案需要1-2周才能完成。
Collagen antibody-induced arthritis (CAIA) is a simple mouse model of rheumatoid arthritis that can be used to address questions of pathogenic mechanisms and to screen candidate therapeutic agents. Arthritis is stimulated by the administration of a cocktail of monoclonal antibodies that are directed to conserved auto-antigenic epitopes in collagen type II, followed by endotoxin. The antibody-induced arthritis model offers several key advantages over the classic collagen-induced arthritis (CIA) model. These include rapid disease onset, high uptake rate, synchronicity, and the capacity to use genetically modified mice, such as transgenics and knockouts. This protocol takes 1-2 weeks to be completed.