Nanostructures from Synthetic Genetic Polymers.
Nanostructures from Synthetic Genetic Polymers.
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DOI：
10.1002/cbic.201600136
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发表时间：
2016-06-16
期刊：
影响因子：
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通讯作者：
Holliger P
中科院分区：
文献类型：
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作者：
Taylor AI;Beuron F;Peak-Chew SY;Morris EP;Herdewijn P;Holliger P
越来越复杂的纳米级物体可以由DNA或RNA构建。然而，潜在的应用范围可以通过扩展超出天然核酸的适度化学多样性来增强。在这里，我们探索完全由替代构建块制成的纳米物体的构建：自然界中没有发现的合成遗传聚合物，也称为异种核酸（XNA）。具体来说，我们描述了在四种不同的XNA化学（2′-氟-2 ′-脱氧-呋喃核糖核酸（2′ F-RNA），2′-氟阿拉伯糖核酸（FANA），己糖醇核酸（HNA）和环己烯核酸（CeNA））中精心制作的70 kDa四面体的组装，以及混合设计，以及通过电子显微镜观察到的600 kDa全FANA八面体。 我们的研究结果扩展了可编程纳米结构组装的化学范围，对纳米物体和材料的设计具有广泛的结构和物理化学性质，包括增强的生物稳定性。
Nanoscale objects of increasing complexity can be constructed from DNA or RNA. However, the scope of potential applications could be enhanced by expanding beyond the moderate chemical diversity of natural nucleic acids. Here, we explore the construction of nano‐objects made entirely from alternative building blocks: synthetic genetic polymers not found in nature, also called xeno nucleic acids (XNAs). Specifically, we describe assembly of 70 kDa tetrahedra elaborated in four different XNA chemistries (2′‐fluro‐2′‐deoxy‐ribofuranose nucleic acid (2′F‐RNA), 2′‐fluoroarabino nucleic acids (FANA), hexitol nucleic acids (HNA), and cyclohexene nucleic acids (CeNA)), as well as mixed designs, and a ∼600 kDa all‐FANA octahedron, visualised by electron microscopy. Our results extend the chemical scope for programmable nanostructure assembly, with implications for the design of nano‐objects and materials with an expanded range of structural and physicochemical properties, including enhanced biostability.