Segment boundaries of the adult rat epididymis limit interstitial signaling by potential paracrine factors and segments lose differential gene expression after efferent duct ligation
Segment boundaries of the adult rat epididymis limit interstitial signaling by potential paracrine factors and segments lose differential gene expression after efferent duct ligation
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DOI：
10.1111/j.1745-7262.2007.00302.x
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发表时间：
2007-07-01
影响因子：
2.9
通讯作者：
Finger, Joshua N.
中科院分区：
文献类型：
--
作者：
Turner, Terry T.;Johnston, Daniel S.;Finger, Joshua N.
附睾分为头部、体部和尾部区域，由结缔组织隔（CTS）分隔成区域内段。在成年大鼠和小鼠中，这些节段高度分化。这些部分的调节是由内分泌，光分泌和旁分泌因子，其相对重要性仍在调查中。在这里，审查的能力的CTS限制信号在间质区室的影响是15天的单侧输出管结扎（EDL）对同侧节段性转录谱。表皮生长因子（EGF）、血管内皮生长因子（VEGFA）和成纤维细胞生长因子2（FGF 2）的微灌注可增加大鼠附睾I段和2段丝裂原活化蛋白激酶（MAPK）的磷酸化，且所有因素的作用均受到分隔各段的CTS的限制。节段基因表达的微阵列分析确定了单侧EDL 15天对大鼠节段1-4的全转录组基因表达的影响。超过11 000个基因表达在每个节段和超过2 000个转录在节段I响应睾丸光分泌因子的剥夺。对照组织的片段I和2在转录上差异最大，EDL在那里的作用最大。在没有光泌因子的情况下，所有四个片段都退化到转录未分化状态，与分化程度较低的组织学一致。去荧光素酶因子可以刺激某些片段中单个基因的表达，但抑制其他片段中的表达。这样的结果揭示了更高的复杂性，大鼠附睾节段的调节比一般理解。
The epididymis is divided into caput, corpus and cauda regions, organized into intraregional segments separated by connective tissue septa (CTS). In the adult rat and mouse these segments are highly differentiated. Regulation of these segments is by endocrine, lumicrine and paracrine factors, the relative importance of which remains under investigation. Here, the ability of the CTS to limit signaling in the interstitial compartment is reviewed as is the effect of 15 days of unilateral efferent duct ligation (EDL) on ipsilateral segmental transcriptional profiles. Inter-segmental microperifusions of epidermal growth factor (EGF), vascular endothelial growth factor (VEGFA) and fibroblast growth factor 2 (FGF2) increased phosphorylation of mitogen activated protein kinase (MAPK) in segments I and 2 of the rat epididymis and the effects of all factors were limited by the CTS separating the segments. Microarray analysis of segmental gene expression determined the effect of 15 days of unilateral EDL on the transcriptome-wide gene expression of rat segments 1-4. Over 11 000 genes were expressed in each of the four segments and over 2 000 transcripts in segment I responded to deprivation of testicular lumicrine factors. Segments I and 2 of control tissues were the most transcriptionally different and EDL had its greatest effects there. In the absence of lumicrine factors, all four segments regressed to a transcriptionally undifferentiated state, consistent with the less differentiated histology. Deprivation of lumicrine factors could stimulate an individual gene's expression in some segments yet suppress it in others. Such results reveal a higher complexity of the regulation of rat epididymal segments than that is generally appreciated.