MONOCLONAL-ANTIBODIES FOR DENGUE VIRUS PRM GLYCOPROTEIN PROTECT MICE AGAINST LETHAL DENGUE INFECTION
MONOCLONAL-ANTIBODIES FOR DENGUE VIRUS PRM GLYCOPROTEIN PROTECT MICE AGAINST LETHAL DENGUE INFECTION
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DOI：
10.4269/ajtmh.1989.41.576
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发表时间：
1989-11-01
影响因子：
3.3
通讯作者：
ECKELS, KH
中科院分区：
文献类型：
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作者：
KAUFMAN, BM;SUMMERS, PL;ECKELS, KH
针对登革病毒3型和登革病毒4型的prM糖蛋白的5种鼠单克隆抗体（Mabs）用于被动保护小鼠体内免受同源和异源登革病毒血清型的致死性攻击。5个prM反应性单克隆抗体中有4个交叉保护小鼠免受异源性攻击，而1个仅保护小鼠免受同源血清型的攻击。尽管很容易证明与病毒体的体外结合，但只有2种prM Mab具有可检测的中和活性。抗鼠免疫球蛋白或补体不能增强中和活性。然而，5个prM Mab中的4个固定补体。这是第一次报告prM特异性单克隆抗体在小鼠中具有保护作用。
Five murine monoclonal antibodies (Mabs) reactive against the prM glycoproteins of DEN-3 and -4 were used to passively protect mice in vivo against lethal challenge with homologous and heterologous dengue virus serotypes. Four of the 5 prM-reactive monoclonals cross-protected mice against heterologous challenge, whereas 1 protected against challenge with only the homologous serotype. Although in vitro binding to virions was readily demonstrated, only 2 of the prM Mabs had detectable neutralizing activity. The neutralizing activity could not be enhanced by anti-mouse immunoglobulin or complement. However, 4 of the 5 prM Mabs fixed complement. This is the first report of prM-specific Mabs that are protective in mice.