Culture of one-cell hamster embryos with water soluble vitamins: pantothenate stimulates blastocyst production
Culture of one-cell hamster embryos with water soluble vitamins: pantothenate stimulates blastocyst production
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DOI：
10.1093/humrep/15.1.157
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发表时间：
2000-01-01
影响因子：
6.1
通讯作者：
Bavister, BD
中科院分区：
文献类型：
--
作者：
McKiernan, SH;Bavister, BD
在不含蛋白质的化学成分确定的培养基HECM-6中检查了水溶性维生素单独或组合对仓鼠1-细胞胚胎发育的影响。泛酸显着刺激囊胚发育相比，无维生素的控制和每一个其他单一的维生素，除了硫胺素，抗坏血酸，生物素，胆碱，叶酸？肌醇、烟酰胺、吡哆醛、核黄素和硫胺素对卵裂期发育或桑椹胚/囊胚形成没有可检测的刺激或抑制。当测试维生素的组合时，胚胎发育没有变化或显著大于对照，但从未显著大于单独使用泛酸盐的发育。对泛酸盐的剂量反应表明3 μ mol/l是最佳浓度。胚胎移植后，每100个在HECM-6加泛酸盐（现命名为HECM-9）中培养的1-细胞胚胎中回收的活胎百分比为24%，显著高于100个单独在HECM-6中培养的1-细胞胚胎中回收的11%。这是第一份报告，以显示一个单一的维生素对植入前胚胎在盟友哺乳动物物种的体外发育的刺激作用。
The effects of water-soluble vitamins, singly or in combinations, on development of hamster 1-cell embryos were examined in a protein-free, chemically defined culture medium, HECM-6. Pantothenate significantly stimulated blastocyst development compared to the vitamin-free control and to every other single vitamin, except thiamine, Ascorbic acid, biotin, choline, folic acid? inositol, niacinamide, pyridoxal, riboflavin and thiamine had no detectable stimulation or inhibition on cleavage stage development or morula/blastocyst formation. When combinations of vitamins were tested, embryo development was either unchanged or significantly greater than in the control, but never significantly greater than development with pantothenate alone, A dose response to pantothenate indicated that 3 mu mol/l was the optimum concentration. After embryo transfer, the percentage of live fetuses recovered per 100 1-cell embryos cultured in HECM-6 plus pantothenate (now designated HECM-9) was 24%, significantly higher than the 11% recovered from 100 1-cell embryos cultured in HECM-6 alone. This is the first report to show a stimulatory effect of a single vitamin on in-vitro development of preimplantation embryos in ally mammalian species.