Indoor Use of Plastic Sheeting Impregnated with Carbamate Combined with Long-Lasting Insecticidal Mosquito Nets for the Control of Pyrethroid-Resistant Malaria Vectors vector-resistance management.
Indoor Use of Plastic Sheeting Impregnated with Carbamate Combined with Long-Lasting Insecticidal Mosquito Nets for the Control of Pyrethroid-Resistant Malaria Vectors vector-resistance management.
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室内使用氨基甲酸酯浸渍的塑料布结合长效杀虫蚊帐来控制拟除虫菊酯抗性疟疾病媒病媒抗药性管理。
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发表时间：
2010
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通讯作者：
V. Corbel
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作者：
Armel Djènontin;F. Chandre;K. Dabiré;Joseph Chabi;R. N’guessan;T. Baldet;M. Akogbeto;V. Corbel
.在布基纳法索的实验性棚屋中，对长效驱虫蚊帐和经氨基甲酸酯处理的塑料布或室内滞留喷洒在控制抗药性蚊子方面的综合效果进行了评价。该地区的冈比亚按蚊对拟除虫菊酯有抗药性，对氨基甲酸酯也有抗药性。观察到LLIN（44%）、IRS（42%）和CTPS（52%）的死亡率相对较低，而这两种组合杀死的蚊子明显更多（LLIN + CTPS和LLIN + IRS约70%）。血液喂养安。与LLIN（43%）、LLIN + CTPS（58%）和LLIN + IRS（56%）相比，IRS和CTPS不抑制冈比亚红细胞。没有证据表明选择的kdr和ace-1 R等位基因的组合，而蚊子的生存优势轴承ace-1 R突变的IRS和CTPS观察。研究结果表明，两种干预措施的结合构成了一种潜在的工具，该研究还显示，经拟除虫菊酯处理的蚊帐对拟除虫菊酯耐药性疟疾病媒的性能大幅下降，这证实了先前在贝宁9和科特迪瓦的研究结果。[28]在我们的研究地点（布基纳法索的库谷），在An的分子M型中观察到kdr等位基因频率的快速增加（从0.4到0.9）。冈比亚与以前的抗药性监测相比。如果这种突然增加的原因是未知的，它似乎对LLIN有重大影响，这是由另一个最近的实验小屋试验显示。在我们的研究中，没有观察到特定类型的kdr抗性选择。然而，易感基因型太少而不能得出任何处理（单独或组合）对kdr选择压力的影响的结论（即，等位基因达到固定水平）。在kdr等位基因频率较低的地区应进行进一步的实验性hut试验，以证实或否定这一趋势。关于氨基甲酸酯耐药性，我们证实An分子M形式中存在ace-1 R等位基因（11%）。在谷杜寇。从小屋收集的活蚊子和死蚊子的基因分型显示了在IRS和CTPS处理后存活的蚊子中ace-1 R等位基因频率显著更高的证据（即，ace-1 R杂合子具有生存优势。考虑到正在通过总统倡议30在若干非洲国家扩大使用恶虫威的大规模室内滞留喷洒运动，这一点尤其令人担忧。加上国税局似乎漠不关心地杀死或
. The combined efficacy of a long-lasting insecticidal net (LLIN) and a carbamate-treated plastic sheeting (CTPS) or indoor residual spraying (IRS) for control of insecticide-resistant mosquitoes was evaluated in experimental huts in Burkina Faso. Anopheles gambiae from the area is resistant to pyrethroids and to a lesser extent, carbamates. Relatively low mortality rates were observed with the LLIN (44%), IRS (42%), and CTPS (52%), whereas both combinations killed significantly more mosquitoes (~70% for LLIN + CTPS and LLIN + IRS). Blood feeding by An. gambiae was uninhibited by IRS and CTPS compared with LLIN (43%), LLIN + CTPS (58%), and LLIN + IRS (56%). No evidence for selection of the kdr and ace-1 R alleles was observed with the combinations, whereas a survival advantage of mosquitoes bearing the ace-1 R mutation was observed with IRS and CTPS. The results suggest that the combination of the two interventions constitutes a potential tool for This study also showed a strong decrease in pyrethroid-treated nets performance against pyrethroid-resistant malaria vectors, which confirms previous findings in Benin 9 and Cote d’Ivoire. 28 In our study site (Vallée du Kou, Burkina Faso), a rapid increase in kdr allelic frequency (from 0.4 to 0.9) was observed in the molecular M form of An. gambiae compared with previous resistance monitoring. If the reason for this sudden increase is unknown, it seems to have a significant impact on LLIN, which is shown by another recent experimental hut trial. In our study, no particular type of selection for the kdr resistance was observed. However, the susceptible genotypes were too rare to make conclusions about the effect of any treatment (alone or combined) on the kdr selection pressure (i.e., the allele reached a fixation level). Further experimental hut trials should be carried out in areas with lower kdr allelic frequency to confirm or deny this trend. Regarding carbamate resistance, we confirmed the presence of the ace-1 R allele (11%) in the molecular M form of An. gambiae in the Vallée du Kou. 14 Genotyping of live and dead mosquitoes collected from the huts showed evidence for a significantly higher frequency of ace-1 R allele in those that survived the IRS and CTPS treatments (i.e., ace-1 R heterozygotes showed a survival advantage). This is particularly worrisome considering that large-scale IRS campaigns using bendiocarb are being scaled up in several African countries through the President Initiative 30 Previous hut trials carried out in Cote d’Ivoire showed similar types of selection of the ace-1 R allele the use of carbamate-treated mosquito nets but not pyrethroid and carbamate “two-in-one” combination nets. the plus IRS seemed to indifferently kill the or