The relationship between the dissolved inorganic carbon concentration and growth rate in marine phytoplankton
The relationship between the dissolved inorganic carbon concentration and growth rate in marine phytoplankton
复制标题
DOI：
10.1098/rspb.2000.1096
复制
发表时间：
2000-05-22
影响因子：
4.7
通讯作者：
Flynn, KJ
中科院分区：
文献类型：
--
作者：
Clark, DR;Flynn, KJ
在封闭系统中培养了一系列海洋浮游植物，以研究在恒定pH条件下溶解无机碳（DIC）利用的动力学和氮源的影响。DIC利用的动力学可用矩形双曲线描述，得到K-G（DIC）的估计值。（碳-特定生长的半饱和常数，即C μ）和μ（max）（理论最大C μ）所有物种的K-G（DIC）在30-750 μ M DIC的范围内。对于大多数物种，NH 4+的使用使生长具有较低的K-G（DIC）和/或，对于两个物种，mu（最大值）增加。在DIC浓度> 1.6mM时，相对于天然海水DIC浓度为2.0mM时的速率，所有物种的C mu均> 90%饱和。结果表明，在贫营养海洋的准稳态条件下，DIC不会显著限制初级生产力的速率和程度。该方法需要应用在与动态沿海（富营养化）系统澄清潜在的DIC速率限制，其中细胞可能会生长到更高的密度和可变的pH值和氮供应下的条件。
A range of marine phytoplankton was grown in closed systems in order to investigate the kinetics of dissolved inorganic carbon (DIC) use and the influence of the nitrogen source under conditions of constant pH. The kinetics of DIC use could be described by a rectangular hyperbolic curve, yielding estimations of K-G(DIC) (the half saturation constant for carbon-specific growth, i.e. C mu) and mu(max) (the theoretical maximum C mu) All species attained a K-G(DIC) within the range of 30-750 mu M DIC. For most species, NH4+ use enabled growth with a lower K-G(DIC) and/or, for two species, an increase in mu(max). At DIC concentrations of > 1.6mM, C mu was > 90% saturated for all species relative to the rate at the natural seawater DIC concentration of 2.0 mM. The results suggest that neither the rate nor the extent of primary productivity will be significantly limited by the DIC in the quasi-steady-state conditions associated with oligotrophic oceans. The method needs to be applied in the conditions associated with dynamic coastal (eutrophic) systems for clarification of a potential DIC rate limitation where cells may grow to higher densities and under variable pH and nitrogen supply.