The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis
The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis
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DOI：
10.1101/gad.1342605
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发表时间：
2005-11-01
影响因子：
10.5
通讯作者：
Wu, C
中科院分区：
文献类型：
--
作者：
Badenhorst, P;Xiao, H;Wu, C
DrosophilaBNF是一种含ISWI的ATP依赖的染色质重塑复合物，通过催化核小体滑动来调节转录。为了确定在体内的基因靶点，我们进行了全基因组表达分析的突变体缺乏的cDNAF特异性亚基cDNAF301。引人注目的是，大量蜕皮激素反应靶点被包括在数百个EGF调控基因中。Nurf301突变体不经历幼虫到蛹的变态，并且还增强蜕皮激素受体的显性负突变。此外，纯化的BNF结合EcR蜕皮激素依赖性的方式，这表明它是一个直接效应的核受体活性。在哺乳动物中，BMPF的保守性对类固醇信号传导具有广泛的意义。
Drosophila NURF is an ISWI-containing ATP-dependent chromatin remodeling complex that regulates transcription by catalyzing nucleosome sliding. To determine in vivo gene targets of NURF, we performed whole genome expression analysis on mutants lacking the NURF-specific subunit NURF301. Strikingly, a large set of ecdysone-responsive targets is included among several hundred NURF-regulated genes. Null Nurf301 mutants do not undergo larval to pupal metamorphosis, and also enhance dominant-negative mutations in ecdysone receptor. Moreover, purified NURF binds EcR in an ecdysone-dependent manner, suggesting it is a direct effector of nuclear receptor activity. The conservation of NURF in mammals has broad implications for steroid signaling.