Participation of core histone "tails" in the stabilization of the chromatin solenoid.
Participation of core histone "tails" in the stabilization of the chromatin solenoid.
复制标题
核心组蛋白“尾巴”参与染色质螺线管的稳定化。
DOI：
10.1083/jcb.93.2.285
复制
发表时间：
1982-05
影响因子：
7.8
通讯作者：
Gould, H
中科院分区：
文献类型：
--
作者：
Allan, J;Harborne, N;Rau, D C;Gould, H
我们在此表明，螺线管是由连接组蛋白和核心组蛋白的非球状、高度碱性“尾部”的组合维持的，其在核小体核心的形成中仅发挥次要作用（Whitlock和Simpson，1977.J.Biol.Chem.252：6,516--6,520；Lilley和Tatchell，1977.Nucleic Acids资源。 4:2,039--2,055；以及 Whitlock 和 Stein，1978。J. Biol。化学。 253:3,857--3,861）。含有没有尾部的核心组蛋白的多核小体在有利于螺线管形成的条件下保持基本上未折叠。尾部可以被外来碱性多肽取代，并且在接头组蛋白存在的情况下，螺线管结构随后自发恢复，如通过多种结构标准判断的。由此推论，核心组蛋白尾段通过提供 DNA 电荷的静电屏蔽并同时桥接螺线管中的相邻核小体来发挥作用。我们的结果进一步表明，减少核心组蛋白尾部正电荷的转录后修饰（例如ε氨基的乙酰化）往往会破坏高阶结构的稳定性，从而使与其相关的DNA更容易用于转录。
We show here that the solenoid is maintained by the combination of linker histones and the nonglobular, highly basic "tails" of the core histones, which play only a minor part in the formation of the nucleosome core (Whitlock and Simpson, 1977. J. Biol. Chem. 252:6,516-- 6,520; Lilley and Tatchell, 1977. Nucleic Acids Res. 4:2,039--2,055; and Whitlock and Stein, 1978. J. Biol. Chem. 253:3,857--3,861). Polynucleosomes that contain core histones devoid of tails remain substantially unfolded under conditions otherwise favorable for the formation of solenoids. The tails can be replaced by extraneous basic polypeptides and in the presence of the linker histones the solenoid structure is then spontaneously recovered, as judged by a wide variety of structural criteria. The inference is that the core histone tail segments function by providing electrostatic shielding of the DNA charge and at the same time bridging adjacent nucleosomes in the solenoid. Our results carry the further implication that posttranscriptional modifications, such as acetylation of epsilon-amino groups, that reduce the positive charge of the core histone tails will tend to destabilize the higher-order structure and could thus render the DNA with which they are associated more readily available for transcription.