The Histochemistry of Chronically Sun-Damaged Skin
The Histochemistry of Chronically Sun-Damaged Skin
复制标题
长期晒伤皮肤的组织化学
DOI：
10.1038/jid.1961.141
复制
发表时间：
1961
影响因子：
6.5
通讯作者：
J. G. Smith
中科院分区：
文献类型：
--
作者：
W. Sams;J. G. Smith
方法先对已知含有高浓度酸性粘多糖的组织进行染色，以确定正常染色反应；尤其是空气质量。正常牛韧带、人软骨、角膜、结肠、支气管、主动脉、心瓣膜、脐带标本作为对照组织，在10%福尔马林（pH 6.0）中固定24-48小时，石蜡包埋，切厚6微米。相邻切片放置在单独的显微镜载玻片上，用苏木红和伊红（H和E）、Oreein、Acid Oreein和Giemsa、Alcian蓝、Mowry’s胶体铁染色和AOQ染色。Mowry的方法按照他所概述的方法进行（20），但以核快红作为一种
METHODSTissues known to contain a high concentration of acid mucopolysaccharide were stained first in order to determine normal staining reactions; especially that of AOQ. Specimens of normal bovine ligamentum nuehae, human cartilage, cornea, colon, bronchus, aorta, heart valve, and umbilical cord served as control tissues and were fixed for 24-48 hours in 10% formalin (pH 6.0), embedded in paraffin, and cut 6 microns thick. Adjacent sections were placed on separate micro-scope slides and stained with hematoxyhn and eosin (H and E), Oreein, Acid Oreein and Giemsa, Alcian blue, Mowry's colloidal iron stain, and AOQ. Mowry's method was performed as out-lined by him (20), but with Nuclear Fast Red as a