MicroRNA Profile of Granulosa Cells after Ovarian Stimulation Differs According to Maturity of Retrieved Oocytes
MicroRNA Profile of Granulosa Cells after Ovarian Stimulation Differs According to Maturity of Retrieved Oocytes
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DOI：
10.1055/s-0041-111173
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发表时间：
2016-06-01
影响因子：
2.7
通讯作者：
Choi, Y. M.
中科院分区：
文献类型：
--
作者：
Kim, Y. J.;Ku, S. -Y.;Choi, Y. M.
背景资料：最近的动物研究表明，调节颗粒细胞（GC）中的microRNA（miRNA）调节卵母细胞的减数分裂能力。然而，人类GCs中miRNA表达谱的差异是否与卵母细胞的成熟度有关仍有待阐明。目的：本观察性研究探讨了人类GCs中miRNA表达谱是否与体外受精（IVF）后获得的卵母细胞的成熟度有关。方法：招募了10名接受GnRH激动剂长方案卵巢刺激周期的女性。取卵时分别抽取优势卵泡的卵泡液，根据卵母细胞成熟度将卵母细胞分为“成熟组”和“未成熟组”。GC收集FF和miRNA进行了分析，使用实时PC：。结果：MII卵母细胞的平均数在成熟组为1.6 - 0.9，没有在未成熟组（p = 0.008）。MI卵母细胞的平均数为5.6 Z.1在成熟组和1.0 0.0在未成熟组（p = 0.008）。成熟组的获卵总数为8.8 ± 1.9，未成熟组为2.0 ± 1.2（p = 0.008）。结论：人优势卵泡GCs中hsalet-7 b的表达与邻近卵母细胞的成熟度有关，可作为IVF结局的预测指标。
Background: Recent animal studies demonstrated that regulating the microRNA (miRNA) in granulosa cells (GCs) modulates the meiotic competence of oocytes. However, the difference in expression profiles of miRNAs in human GCs according to the maturity of the oocyte still remains to be elucidated.Objective: This observational study investigated whether the miRNA profile of human GCs differs according to the maturity of the retrieved oocyte after controlled ovarian stimulation for in vitro fertilization (IVF).Methods: Ten women who underwent ovarian stimulation cycles with GnRH agonist long protocols were recruited. The follicular fluid (FF) from dominant follicles was individually aspirated at oocyte retrieval, Oocytes were divided into two groups according to oocyte maturity ("mature group" vs. "immature group"). GCs were collected from the FF and miRNA was analyzed using realtime PC:.Results: Mean number of MII oocytes in the mature group was 1.6 0.9 with none in the immature group (p = 0.008). Mean number of MI oocytes was 5.6 Z.1 in the mature group and 1.0 0.0 in the immature group (p = 0.008). The total number of retrieved oocytes was 8.8 1.9 in the mature group and 2.0 1,2 in the immature group (p = 0.008). The GCs of the mature group showed a significantly lower expression of hsalet-7b compared to the GCs of the immature group (p < 0.001).Conclusion: Taken together, the miRNA expression profiles of human GCs obtained from dominant follicles are associated with maturity of the adjacent oocyte and may be useful as a prognosticator of IVF outcome.