QTL mapping in testcrosses of European flint lines of maize .1. Comparison of different testers for forage yield traits
QTL mapping in testcrosses of European flint lines of maize .1. Comparison of different testers for forage yield traits
复制标题
DOI：
10.2135/cropsci1997.0011183x003700030037x
复制
发表时间：
1997-05-01
期刊：
影响因子：
2.3
通讯作者：
Klein, D
中科院分区：
文献类型：
--
作者：
Lubberstedt, T;Melchinger, AE;Klein, D
在世界较冷的温带地区，玉米（Zea mays L.）在这项研究中，我们定位和表征数量性状基因座（QTL）影响测交性能的重要饲用玉米性状，并调查其在不同环境和测试人员的一致性。以2个优良的燧石自交系为亲本，通过杂交获得380个F2代单株，其中345个单株在89个RFLP（restriction fragment length polymorphism）位点上进行了基因型分析。每个测交系列在5个环境下进行2个重复的田间试验。分析了5个性状：干物质产量（DMY）、干物质浓度（DMC）、株高（PHT）、淀粉浓度（STC）和淀粉产量（STY）。基因型（和基因型×环境方差是非常显着的所有性状在两个TC系列。除STY（0.46，0.47）外，其余性状的遗传力在0.64 ~ 0.88之间，各性状间的基因型相关均大于0.80，QTL分析采用复合区间作图法。在每个测交系列中检测到7个（STY）和16个（PHT）QTL，解释了52%和71%的同时拟合，少数QTL表现出与环境的显着互作。结果是高度一致的测试者之间的DMC和PHT，但不是DMY，STC，和STY，虽然TC世代的手段比较表明显着上位性DMY和PHT，QTL之间的显着相互作用检测到在两种情况下。如果一个基因组区域同时影响两个性状，则在大多数情况下，正相关性状的个体性状的等位基因增加来自同一亲本，而负相关性状的个体性状的等位基因增加来自不同亲本。
In the cooler temperate regions of the world, maize (Zea mays L.) is primarily cultivated for forage usage, In this study, we mapped and characterized quantitative trait loci (QTL) affecting testcross performance of important forage maize traits and investigated their consistency across environments and testers. Two elite flint inbred lines were crossed to generate 380 F-2 individuals, 345 of which were genotyped at 89 RFLP (restriction fragment length polymorphism) marker loci, The 380 F-3 lines obtained by selfing the F-2 individuals and the parent lines were crossed to two diverse dent inbred testers. Each series of testcrosses (TC) was evaluated in field trials with two replicates in five environments, Five traits were analyzed: dry matter yield (DMY), dry matter concentration (DMC), plant height (PHT), starch concentration (STC), and starch yield (STY). Genotypic ( and genotype x environment variances were highly significant for all traits in both TC series. Heritabilities ranged from 0.64 to 0.88 except for STY (0.46, 0.47), Genotypic correlations between testers were high (> 0.80) for all traits, QTL analyses were performed by composite interval mapping. Between seven (STY) and 16 (PHT) QTL were detected in each testcross series, explaining between 52 and 71% of in a simultaneous fit, Few QTL displayed significant interactions with environments. Results were highly consistent across testers for DMC and PHT, but not for DMY, STC, and STY, Although a comparison of TC generation means indicated significant epistasis for DMY and PHT, significant interactions between QTL were detected in two cases only. If a genomic region simultaneously affected two characters, the increasing alleles for the individual traits originated in most cases from the same parent for positively correlated traits and from different parents for negatively correlated traits.