Thiol profiling in cancer cell lines by HPLC-mass spectrometry.
Thiol profiling in cancer cell lines by HPLC-mass spectrometry.
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DOI：
10.1016/j.xpro.2021.100977
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Gan B
中科院分区：
文献类型：
--
作者：
Liu X;Koppula P;Olszewski K;Gan B
我们描述了一种使用高效液相色谱（HPLC）串联质谱法鉴定贴壁细胞中半胱氨酸和胱氨酸等细胞硫醇代谢物的方案。我们采用改进的提取和样品衍生化方案来准确量化细胞内不稳定硫醇物种的水平，并在分析前抑制氧化。有关使用和执行本协议的完整详情，请参阅和。基于lc - ms的不稳定硫醇物种的定量改进提取和衍生过程以防止样品氧化我们描述了一种基于高效液相色谱（HPLC）串联质谱的代谢组学方法，用于鉴定粘附细胞中的细胞硫醇代谢物，如半胱氨酸和胱氨酸。我们采用改进的提取和样品衍生化方案来准确量化细胞内不稳定硫醇物种的水平，并在分析前抑制氧化。
We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to and. LC-MS-based quantification of labile thiol species Modified extraction and derivatization procedure to prevent sample oxidation Protocol for quantification of reduced and oxidized thiol species We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis.
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影响因子：
5.8
作者：
Koppula P;Olszewski K;Zhang Y;Kondiparthi L;Liu X;Lei G;Das M;Fang B;Poyurovsky MV;Gan B
通讯作者：
Gan B
影响因子：
4.3
作者：
Sporea, Ioan;Popescu, Alina;Vernic, Corina
通讯作者：
Vernic, Corina
影响因子：
7.4
作者：
Inoue, T;Kirchhoff, JR
通讯作者：
Kirchhoff, JR
影响因子：
64.5
作者：
Chen, Walter W.;Freinkman, Elizaveta;Wang, Tim;Birsoy, Kivanc;Sabatini, David M.
通讯作者：
Sabatini, David M.
影响因子：
7.4
作者：
Lu, Wenyun;Clasquin, Michelle F.;Melamud, Eugene;Amador-Noguez, Daniel;Caudy, Amy A.;Rabinowitz, Joshua D.
通讯作者：
Rabinowitz, Joshua D.