Optical observation of DNA translocation through Al2O3 sputtered silicon nanopores in porous membrane
Optical observation of DNA translocation through Al2O3 sputtered silicon nanopores in porous membrane
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DOI：
10.1007/s00339-016-9764-9
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
Hirohito Yamazaki;Shintaro Ito;K. Esashika;Y. Taguchi;T. Saiki
中科院分区：
文献类型：
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作者：
Hirohito Yamazaki;Shintaro Ito;K. Esashika;Y. Taguchi;T. Saiki
纳米孔传感器正在被开发为分析单个 DNA、RNA 和蛋白质的平台。在纳米孔传感器中，离子电流测量被广泛使用，并且先前的研究已经证明了通过它进行纳米孔 DNA 测序的概念验证。最近，我们提出了一种纳米孔 DNA 测序的替代平台，该平台结合了紫外光和多孔硅膜来执行高通量测量。在我们的 DNA 测序平台的开发中，控制多孔硅膜中的纳米孔尺寸至关重要，但仍然是一个挑战。在这里，我们报告了通过我们的光学方案观察到的 DNA 易位通过 Al2O3 溅射硅纳米孔（Al2O3 纳米孔）的情况。通过电磁波模拟分析聚焦紫外光产生的 Al2O3 纳米孔的激发体积。实验中，比较了DNA通过Al2O3纳米孔与硅纳米孔的转位时间，并通过补充静电场模拟来检验纳米孔密度和膜厚度的影响。
Nanopore sensors are being developed as a platform for analyzing single DNA, RNA, and protein. In nanopore sensors, ionic current measurement is widely used and proof-of-concept of nanopore DNA sequencing by it has been demonstrated by previous studies. Recently, we proposed an alternative platform of nanopore DNA sequencing that incorporates ultraviolet light and porous silicon membrane to perform high-throughput measurement. In the development of our DNA sequencing platform, controlling nanopore size in porous silicon membrane is essential but remains a challenge. Here, we report on observation of DNA translocation through Al2O3sputtered silicon nanopores (Al2O3nanopores) by our optical scheme. Electromagnetic wave simulation was performed to analyze the excitation volume on Al2O3nanopores generated by focused ultraviolet light. In the experiment, DNA translocation time through Al2O3nanopores was compared with that of silicon nanopores and we examined the effect of nanopore density and thickness of membrane by supplementing the static electric field simulation.