STRUCTURAL REQUIREMENTS OF ADENOVIRUS VAI RNA FOR ITS TRANSLATION ENHANCEMENT FUNCTION
STRUCTURAL REQUIREMENTS OF ADENOVIRUS VAI RNA FOR ITS TRANSLATION ENHANCEMENT FUNCTION
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DOI：
10.1128/mcb.5.1.187
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
THIMMAPPAYA, B
中科院分区：
文献类型：
--
作者：
BHAT, RA;DOMER, PH;THIMMAPPAYA, B
最近，通过遗传学和生物化学方法，显示腺病毒VAI RNA是在感染后晚期有效翻译病毒mRNA种类所必需的。为了了解负责VAI RNA在增强翻译中的作用的VAI RNA的核苷酸序列和结构域，进行了VAI基因的突变分析。在质粒水平上将覆盖VAI RNA的大部分核苷酸序列的缺失、取代和插入突变引入VAI基因。然后将这些突变基因重新导入病毒中，研究突变病毒的生长特性，发现大多数突变体保留了正常或接近正常的生物学功能。在+43和+53之间以及+107和基因的3“末端之间的区域中的突变导致活性的相当大的损失。然而，这些突变体的生长明显优于缺乏功能性VAI和VAII基因的5型腺病毒突变体，表明它们保留了部分活性。由于没有1个突变能够完全消除该功能，因此VAI RNA可能具有多个功能位点用于其翻译调节功能。这些多个位点可以是短的寡核苷酸序列，其可以在翻译期间与细胞和/或病毒组分相互作用。
Recently, by genetic and biochemical approaches, it was shown that adenovirus VAI RNA is required for efficient translation of viral mRNA species at late times after infection. To understand the nucleotide sequences and the domains of the VAI RNA that are responsible for the role of VAI RNA in enhancement of translation, a mutational analysis of the VAI gene was undertaken. Deletion, substitution and insertion mutations covering most of the nucleotide sequences of VAI RNA were introduced into the VAI gene at the plasmid level. These mutant genes were then reintroduced into the virus, and growth properties of the mutant viruses were studied.The majority of the mutants retained normal or nearly normal levels of biological function. Mutations in the region between +43 and +53 and between +107 and the 3'' end of the gene resulted in a considerable loss of activity. These mutants, however, grew significantly better than did an adenovirus type 5 mutant lacking both functional VAI and VAII genes, indicating that they retain a portion of their activity. Because no 1 mutation was able to completely abolish the function, the VAI RNA may have multiple functional sites for its translation modulation function. These multiple sites may be short oligonucleotide sequences that may interact with cellular and/or viral components during translation.