MOLECULAR ANALYSIS OF SMOOTH-MUSCLE DEVELOPMENT IN THE MOUSE
MOLECULAR ANALYSIS OF SMOOTH-MUSCLE DEVELOPMENT IN THE MOUSE
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DOI：
10.1002/aja.1002040306
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发表时间：
1995-11-01
影响因子：
2.5
通讯作者：
MCHUGH, KM
中科院分区：
文献类型：
--
作者：
MCHUGH, KM
目前对于正常哺乳动物发育过程中平滑肌组织的个体发育知之甚少。 α-平滑肌和γ-平滑肌同肌动蛋白已被证明是平滑肌细胞表型的优异分子标记。这项研究描述了发育中小鼠中 or-平滑肌和 γ-平滑肌异肌动蛋白表达的时间和空间模式。使用α-平滑肌和γ-平滑肌同肌动蛋白特异性核糖探针，对胚胎第9、11、13、15和17天的整个小鼠胚胎的连续切片进行原位分析。在发育中的胃肠道、泌尿生殖道、呼吸道和血管系统中观察到α-平滑肌和γ-平滑肌同肌动蛋白基因表达的不同时间和空间模式。在骨骼肌、心脏和平滑肌肌发生的早期阶段以及在不同器官的新子集中（包括后肠、尿囊和原始胎盘的肛后部分）观察到平滑肌异肌动蛋白的独立表达。这项研究的结果表明，不同的细胞表型参与平滑肌肌发生，并表明可能存在启动体内平滑肌发育的器官特异性机制。此外，本研究中观察到的独立或平滑肌异肌动蛋白表达模式提供了有关后肠和胎盘发育早期阶段的新信息，并表明常见的功能表型可能与骨骼肌、心脏和平滑肌肌发生的早期阶段相关。 (C) 1995 Wiley-Liss, Inc.
Little is currently known regarding the ontogeny of smooth muscle tissues during normal mammalian development. The a-smooth muscle and gamma-smooth muscle isoactins have been shown to be excellent molecular markers of smooth muscle cell phenotype. This study characterizes both the temporal and spatial patterns of or-smooth muscle and gamma-smooth muscle isoactin expression in the developing mouse. In situ analysis was performed on serial sections of whole mouse embryos on embryonic day 9, 11, 13, 15, and 17 using alpha-smooth muscle and gamma-smooth muscle isoactin-specific riboprobes. Distinct temporal and spatial patterns of a-smooth muscle and gamma-smooth muscle isoactin gene expression were observed in the developing gastrointestinal tract, urogenital tract, respiratory tract, and vascular system. Independent expression of the or-smooth muscle isoactin was observed during the early stages of skeletal, cardiac, and smooth muscle myogenesis as well as in a novel subset of distinct organs including the postanal component of the hindgut, allantois, and primitive placenta. The results of this study indicate that distinct cellular phenotypes are involved in smooth muscle myogenesis and suggest that organ-specific mechanisms might exist for the initiation of smooth muscle development in vivo. In addition, the pattern of independent or-smooth muscle isoactin expression observed in this study provides novel information regarding the early stages of hindgut and placental development, and suggests that a common functional phenotype may be associated with the early stages of skeletal, cardiac, and smooth muscle myogenesis. (C) 1995 Wiley-Liss, Inc.