Enhanced saturated fatty acids accumulation in cultures of newly-isolated strains of Schizochytrium sp and Thraustochytriidae sp for large-scale biodiesel production
Enhanced saturated fatty acids accumulation in cultures of newly-isolated strains of Schizochytrium sp and Thraustochytriidae sp for large-scale biodiesel production
复制标题
用于大规模生物柴油生产的新分离的裂殖壶菌属和破囊壶菌科菌株培养物中饱和脂肪酸的积累增强
DOI：
10.1016/j.scitotenv.2018.03.078
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发表时间：
2018
影响因子：
9.8
通讯作者：
Wang Guangyi
中科院分区：
文献类型：
--
作者：
Wang Qiuzhen;Sen Biswarup;Liu Xianhua;He Yaodong;Xie Yunxuan;Wang Guangyi
Heterotrophic marine protists (Thraustochytrids) have received increasingly global attention as a renewable, sustainable and alternative source of biodiesel because of their high ability of saturated fatty acids (SFAs) accumulation. Yet, the influence of extrinsic factors (nutrients and environmental conditions) on thraustochytrid culture and optimal conditions for high SFAs production are poorly described. In the present study, two different thraustochytrid strains,Schizochytriumsp. PKU#Mn4 andThraustochytriidaesp. PKU#Mn16 were studied for their growth and SFAs production profiles under various conditions (carbon, nitrogen, temperature, pH, KH2PO4, salinity, and agitation speed). Of the culture conditions, substrates (C and N) source and conc., temperature, and agitation speed significantly influenced the cell growth and SFAs production of both strains. Although both the strains were capable of growth and SFAs production in the broad range of culture conditions, their physiological responses to KH2PO4, pH, and salinity were dissimilar. Under their optimal batch culture conditions, peak SFAs productions of 3.3 g/L and 2.2 g/L with 62% and 49% SFAs contents (relative to total fatty acids) were achieved, respectively. The results of 5-L fed-batch fermentation under optimal conditions showed a nearly 4.5-fold increase in SFAs production (i.e., 7.5 g/L) by both strains compared to unoptimized conditions. Of the two strains, the quality of biodiesel produced from the fatty acids of PKU#Mn4 met the biodiesel standard defined by ASTM6751. This study, to the knowledge of the authors, is the first comprehensive report of optimal fermentation conditions demonstrating enhanced SFAs production by strains belonging to two different thraustochytrid genera and provides the basis for large-scale biodiesel production.
