COMPUTER-ASSISTED ANALYSIS OF INTERLABORATORY AMES TEST VARIABILITY
COMPUTER-ASSISTED ANALYSIS OF INTERLABORATORY AMES TEST VARIABILITY
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DOI：
10.1080/15287398809531194
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发表时间：
1988-01-01
期刊：
影响因子：
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通讯作者：
GIULIANI, A
中科院分区：
文献类型：
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作者：
BENIGNI, R;GIULIANI, A
通过比较国际致癌物短期试验评价计划（IPESTTC）框架内生成的12组结果，研究了沙门氏菌/微粒体试验的实验室间艾姆斯试验变异性。在整个化学品范围内同时分析测试性能相似性的策略涉及使用多变量数据分析方法。对各组艾姆斯试验数据进行了相互对比，并与选择的其他IPESTTC试验进行了对比。选择这些测试是因为它们代表了对化学品的各种不同反应模式。这种方法使我们既能估计艾姆斯试验的实验室间变异性的绝对程度，又能将其变异性范围与试验反应的总体分布进行对比。12个实验室中有10个实验室表现出高度的实验重现性;两个实验室产生了明显不同的结果，可能与代谢活化方案的差异有关。分析还表明，大肠埃希菌WP 2和中国仓鼠卵巢细胞染色体畸变等检测试剂产生的结果组在沙门氏菌的变异性范围内;从这个意义上说，它们与沙门氏菌不互补。
The interlaboratory Ames test variability of the Salmonella/microsome assay was studied by comparing 12 sets of results generated in the frame of the International Program for the Evaluation of Short-Term Tests for Carcinogens (IPESTTC). The strategy for the simultaneous analysis of test performance similarities over the whole range of chemicals involved the use of multivariate data analysis methods. The various sets of Ames test data were contrasted both against each other and against a selection of other IPESTTC tests. These tests were chosen as representing a wide range of different patterns of response to the chemicals. This approach allowed us both to estimate the absolute extent of the interlaboratory variabilitiy of the Ames test, and to contrast its range of variability with the overall spread of test responses. Ten of the 12 laboratories showed a high degree of experimental reproducibility; two laboratories generated clearly differentiated results, probably related to differences in the protocol of metabolic activation. The analysis also indicated that assays such as Escherichia coli WP2 and chromosomal aberrations in Chinese hamster ovary cells generated sets of results within the variability range of Salmonella; in this sense they were not complementary to Salmonella.