Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate.
Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate.
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DOI：
10.1261/rna.045310.114
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Siomi H
中科院分区：
文献类型：
--
作者：
Hirano T;Iwasaki YW;Lin ZY;Imamura M;Seki NM;Sasaki E;Saito K;Okano H;Siomi MC;Siomi H
动物种系中的 Piwi-piRNA 复合物抑制转座元件 (TE) 以维持基因组完整性。然而，人们对灵长类动物 PIWI-piRNA 通路知之甚少。在本文中，作者分析了普通狨猴（一种模式灵长类动物）的成年睾丸中的 Piwi-piRNA 复合物。他们发现狨猴 piRNA 显示出小鼠粗线期 piRNA 的特征，并且大部分源自保守的 piRNA 簇，其中一些包含具有反义方向的假基因。 In addition, more piRNAs map to TE subfamilies when they have copies in piRNA clusters.他们的研究结果表明，粗线期 piRNA 簇在 TE 陷阱和蛋白质编码基因表达的调节中发挥作用。小RNA通过结合Argonaute/Piwi蛋白来调节靶RNA来介导基因沉默。在这里，我们描述了普通狨猴 Callithrix jacchus 成年睾丸的小 RNA 分析。成人睾丸中最丰富的一类小 RNA 是 piRNA，尽管也鉴定出了 353 个新的 miRNA，但内切 siRNA 很少。 MARWI 是小鼠 MIWI 的狨猴同源物，也是成年睾丸中非常丰富的 PIWI，与显示小鼠粗线期 piRNA 特征的 piRNA 相关。与其他哺乳动物一样，大多数狨猴 piRNA 源自基因组中的保守聚集区域，这些区域被注释为基因间区域。然而，与小鼠不同的是，狨猴的 piRNA 簇也存在于 X 染色体上，这表明 X 连锁簇可以避免减数分裂性染色体失活。一些鉴定出的 piRNA 簇含有反义导向的假基因，这表明假基因衍生的 piRNA 可能调节亲本功能蛋白编码基因。当更多的 piRNA 在 piRNA 簇中有拷贝时，它们会映射到转座元件 (TE) 亚家族。此外，针对映射到每个 TE 亚家族的 piRNA 观察到的链偏向与簇中插入的拷贝的极性相关。这些发现表明，粗线期 piRNA 簇决定了 TE 衍生的 piRNA 的丰度和链偏向，可能通过假基因衍生的 piRNA 调节蛋白质编码基因，甚至可能在成年狨猴睾丸的减数分裂中发挥作用。
The Piwi-piRNA complex in animal germlines represses transposable elements (TEs) to maintain genome integrity. However, little is known about primate PIWI-piRNA pathways. In this paper, the authors analyze Piwi-piRNA complexes in the adult testes of the common marmoset, a model primate. They find that marmoset piRNAs show characteristics of mouse pachytene piRNAs and mostly derive from conserved piRNA clusters, some of which contain pseudogenes with antisense orientation. In addition, more piRNAs map to TE subfamilies when they have copies in piRNA clusters. Their findings suggest that pachytene piRNA clusters function in both TE traps and regulation of protein-coding gene expression. Small RNAs mediate gene silencing by binding Argonaute/Piwi proteins to regulate target RNAs. Here, we describe small RNA profiling of the adult testes of Callithrix jacchus, the common marmoset. The most abundant class of small RNAs in the adult testis was piRNAs, although 353 novel miRNAs but few endo-siRNAs were also identified. MARWI, a marmoset homolog of mouse MIWI and a very abundant PIWI in adult testes, associates with piRNAs that show characteristics of mouse pachytene piRNAs. As in other mammals, most marmoset piRNAs are derived from conserved clustered regions in the genome, which are annotated as intergenic regions. However, unlike in mice, marmoset piRNA clusters are also found on the X chromosome, suggesting escape from meiotic sex chromosome inactivation by the X-linked clusters. Some of the piRNA clusters identified contain antisense-orientated pseudogenes, suggesting the possibility that pseudogene-derived piRNAs may regulate parental functional protein-coding genes. More piRNAs map to transposable element (TE) subfamilies when they have copies in piRNA clusters. In addition, the strand bias observed for piRNAs mapped to each TE subfamily correlates with the polarity of copies inserted in clusters. These findings suggest that pachytene piRNA clusters determine the abundance and strand-bias of TE-derived piRNAs, may regulate protein-coding genes via pseudogene-derived piRNAs, and may even play roles in meiosis in the adult marmoset testis.
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影响因子：
4.3
作者：
Giordano J;Ge Y;Gelfand Y;Abrusán G;Benson G;Warburton PE
通讯作者：
Warburton PE
影响因子：
14.9
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Flicek P;Ahmed I;Amode MR;Barrell D;Beal K;Brent S;Carvalho-Silva D;Clapham P;Coates G;Fairley S;Fitzgerald S;Gil L;García-Girón C;Gordon L;Hourlier T;Hunt S;Juettemann T;Kähäri AK;Keenan S;Komorowska M;Kulesha E;Longden I;Maurel T;McLaren WM;Muffato M;Nag R;Overduin B;Pignatelli M;Pritchard B;Pritchard E;Riat HS;Ritchie GR;Ruffier M;Schuster M;Sheppard D;Sobral D;Taylor K;Thormann A;Trevanion S;White S;Wilder SP;Aken BL;Birney E;Cunningham F;Dunham I;Harrow J;Herrero J;Hubbard TJ;Johnson N;Kinsella R;Parker A;Spudich G;Yates A;Zadissa A;Searle SM
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Searle SM
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10.5
作者：
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44.1
作者：
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通讯作者：
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影响因子：
64.5
作者：
Brennecke, Julius;Aravin, Alexei A.;Hannon, Gregory J.
通讯作者：
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