Zone electrophoresis of proteins in poly(dimethylsiloxane) (PDMS) microchip coated with physically adsorbed amphiphilic phospholipid polymer
Zone electrophoresis of proteins in poly(dimethylsiloxane) (PDMS) microchip coated with physically adsorbed amphiphilic phospholipid polymer
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DOI：
10.1007/s10404-012-1102-8
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发表时间：
2013-06
影响因子：
2.8
通讯作者：
Kyosuke Nii;K. Sueyoshi;K. Otsuka;M. Takai
中科院分区：
文献类型：
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作者：
Kyosuke Nii;K. Sueyoshi;K. Otsuka;M. Takai
研究了以物理吸附两亲性磷脂聚合物（PMMSi）为涂层的聚二甲基硅氧烷（PDMS）微芯片中蛋白质的区带电泳。PMMSi由2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）和3-（甲基丙烯酰氧基）丙基三（三甲基硅氧基）硅烷（MPTSSi）单元以无规方式组成。采用简单、快速的浸涂法在PDMS表面形成PMMSi膜。通过接触角测量、衰减全反射傅立叶变换红外吸收光谱（ATR-FTIR）和X射线光电子能谱（XPS）分析，表明该膜具有较高的亲水性和良好的水稳定性。通过PMMSi涂层实现了对蛋白质吸附到PDMS表面的高度抑制和电渗流（electroosmotic flow，EEP）的减少，这是由于PDMS表面上的亲水性增加和EEP-电位降低。对于区带电泳而言，就在PDMS表面上的涂层膜的稳定性而言，含有30%亲水MPC的PMMSi 30是最合适的分子设计。PMMSi 30包覆的PDMS微芯片的电导率平均值为1.4 × 10− 4cm 2 V − 1 s −1，相对标准偏差为4.1%。进一步证明了铀氨酸的区带电泳具有较高的重复性和重现性。与未经处理的PDMS微芯片相比，两种FITC标记的蛋白质（BSA和胰岛素）的分离效率和分辨率都很高。
The zone electrophoresis of protein in poly(dimethylsiloxane) (PDMS) microchip coated with the physically adsorbed amphiphilic phospholipid polymer (PMMSi) was investigated. PMMSi was composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) and 3-(methacryloyloxy) propyltris (trimethylsiloxy) silane (MPTSSi) units in a random fashion. The membrane of PMMSi can be formed on the PDMS surface by a simple and quick dip-coating method. The membrane showed high hydrophilicity and good stability in water, as determined by contact angle measurement, fourier-transformed infrared absorption by attenuated total reflection (ATR-FTIR), and X-ray photoelectron spectroscopy (XPS) analysis. High suppression of protein adsorption to the PDMS surface and reduction in electroosmotic flow (EOF) were achieved by PMMSi coating due to an increase of hydrophilicity, and a decrease of the ζ-potential on the surface of PDMS. For zone electrophoresis, the PMMSi30 containing 30 % hydrophilic MPC was the most suitable molecular design in terms of the stability of the coated membrane on PDMS surface. The average value of EOF mobility of PDMS microchip coated with PMMSi30 was 1.4 × 10−4cm2V−1s−1, and the RSD was 4.1 %. Zone electrophoresis of uranine was further demonstrated with high repeatability and reproducibility. Separation of two FITC-labeled proteins (BSA and insulin) was performed with high efficiency and resolution compared with non-treated PDMS microchip.