Impact of low level laser irradiation on infarct size in the rat following myocardial infarction
Impact of low level laser irradiation on infarct size in the rat following myocardial infarction
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DOI：
10.1016/s0167-5273(01)00503-4
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发表时间：
2001-09-01
影响因子：
3.5
通讯作者：
Oron, U
中科院分区：
文献类型：
--
作者：
Ad, N;Oron, U
低能量水平辐射（LLLI）已被发现调节生物过程。在慢性结扎大鼠冠状动脉左前降支（LAD）后，观察了LLLI对急性心肌梗死（MI）发生的影响。在MI后和MI后第3天，使用二极管激光器（804 nm，38 mW功率输出）通过胸部的肋间肌对22只大鼠的心脏进行激光照射（LI）。非激光照射对照组（NLI）19只，实验性诱发心肌梗死，不进行激光照射。MI后21天处死所有大鼠。使用组织形态计量学方法，从用Masson三色染色的组织切片和用氯化三苯基四氮唑（TTC）染色的心脏中确定梗死的大小、厚度和相对周长以及其他参数。LI大鼠的梗死面积（表示为左心室总面积的百分比）为10.1 ± 5.8，显著低于（65%; P < 0.01）NLI大鼠的梗死面积（28.7 ± 9.6）。相关地，LI大鼠的梗死区周长比显著低于NLI大鼠（2倍; P < 0.01）。在正确的能量、持续时间和时机下，对实验诱导的MI大鼠心肌中的梗塞区域进行LLLI，显著减少心肌组织的损失。这种现象可能对急性心肌梗死或缺血性心脏病后的患者有重要的有益影响。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Low energy level irradiation (LLLI) has been found to modulate biological processes. The effect of LLLI on the development of acute myocardial infarction (MI) was investigated following chronic ligation of the left anterior descending (LAD) coronary artery in laboratory rats. The hearts of 22 rats were laser irradiated (LI) using a diode laser (804 nm, 38 mW power output) through the intercostal muscles in the chest following MI and on day 3 post MI. In the control non laser irradiated (NLI) group (19 rats) MI was induced experimentally and laser irradiation was not applied. All rats were sacrificed 21 days post MI. Size, thickness and relative circumferential length of the infarct, as well as other parameters, were determined from histological sections stained with Masson's trichrome and hearts stained with triphenyl tetrazolium chloride (TTC) using histomorphometric methods. The infarct size (expressed as percent of total left ventricle area) of the LI rats was 10.1 +/- 5.8, which was significantly lower (65%; P < 0.01) than the infarct size of NLI rats which was 28.7 +/- 9.6. Correlatively, the ratio of circumferential length of the infarcted area was significantly lower (2-fold; P < 0.01) in the LI rats as compared to the NLI rats. LLLI of the infarcted area in the myocardium of experimentally induced MI rats, at the correct energy, duration and timing, markedly reduces the loss of myocardial tissue. This phenomenon may have an important beneficial effect on patients after acute MI or ischemic heart disease. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.