Enhancement of Satellite Cell Transplantation Efficiency by Leukemia Inhibitory Factor.
Enhancement of Satellite Cell Transplantation Efficiency by Leukemia Inhibitory Factor.
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卫星细胞移植效率提高白血病抑制因子。
DOI：
10.3233/jnd-160156
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发表时间：
2016-05-27
影响因子：
3.3
通讯作者：
Takeda S
中科院分区：
文献类型：
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作者：
Ito N;Shimizu N;Tanaka H;Takeda S
背景和目的：细胞移植是治疗包括杜氏肌营养不良症在内的多种肌肉疾病的一种很有前途的方法。卫星细胞是骨骼肌中的干细胞，为移植治疗提供了重要的细胞来源。然而，卫星细胞的体外培养会导致它们失去未分化状态，从而降低移植效率。因此，有必要开发最佳培养条件以维持卫星细胞的未分化状态。方法：原代卫星细胞培养加或不加白血病抑制因子（LIF）。分析未分化和分化标记物的表达及移植效率。结果如下：LIF处理的卫星细胞显示Pax7表达增加，并在Duchenne肌营养不良症小鼠模型中提高移植效率。结论：我们的研究表明，LIF处理有效地维持了卫星细胞的未分化状态，并提高了其移植效率。这些结果将有助于优化细胞移植治疗的培养条件。
Background and Objectives: Cell transplantation is a promising therapy for several muscle diseases, including Duchenne muscular dystrophy. Satellite cells are stem cells in skeletal muscle that provide an important cell source for transplantation therapy. However, culture of satellite cells in vitro causes them to lose their undifferentiated state, associated with reduced transplantation efficiency. It is therefore necessary to develop optimal culture conditions for maintaining the undifferentiated state of satellite cells. Methods: Primary satellite cells were cultured with or without leukemia inhibitory factor (LIF). The expression of undifferentiation and differentiation markers, and the transplantation efficiency were analyzed. Results: LIF-treated satellite cells showed increased expression of Pax7, and enhanced transplantation efficiency in a mouse model of Duchenne muscular dystrophy. Conclusions: Our study showed that the treatment with LIF effectively maintained the undifferentiated state of satellite cells, and enhanced their transplantation efficiency. These results will contribute to the optimization of culture conditions for cell transplantation therapy.