A plant outer mitochondrial membrane protein with high amino acid sequence identity to a chloroplast protein import receptor
A plant outer mitochondrial membrane protein with high amino acid sequence identity to a chloroplast protein import receptor
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DOI：
10.1016/s0014-5793(03)01457-1
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发表时间：
2004-01-16
期刊：
影响因子：
3.5
通讯作者：
Whelan, J
中科院分区：
文献类型：
--
作者：
Chew, O;Lister, R;Whelan, J
我们在拟南芥线粒体外膜上发现了一种新的蛋白质。该蛋白与叶绿体外膜 (Toc) 的 64 kDa 转位酶具有 67% 的序列同一性。该蛋白的线粒体定位是通过以下因素确定的：(i) 它在高度纯化的拟南芥线粒体的蛋白质组中的存在，(ii) 使用来自豌豆的 Toc64 抗体进行蛋白质印迹分析，表明其存在于拟南芥和豌豆线粒体中，(iii) 绿色荧光蛋白融合蛋白，表明该蛋白的独特线粒体定位，以及 (iv) 表达 各种组织类型和发育过程中的特征与叶绿体 Toc 成分相比更类似于线粒体外膜成分的转位酶。因此，拟南芥线粒体含有与质体蛋白输入受体具有高度序列同一性的蛋白。 (C) 2003 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
We have identified a novel protein on the outer membrane of Arabidopsis thaliana mitochondria. This protein displays 67% sequence identity with the 64 kDa translocase of the outer envelope membrane of chloroplasts (Toc). A mitochondrial localisation for this protein was determined by (i) its presence in the proteome of highly purified Arabidopsis mitochondria, (ii) Western blot analysis with antibodies to Toc64 from pea that indicate its presence in Arabidopsis and pea mitochondria, (iii) green fluorescent protein fusion proteins that indicate an exclusive mitochondrial localisation for this protein, and (iv) expression profiles in various tissue types and during development that are more similar to translocase of the outer mitochondrial membrane components than to chloroplastic Toc components. Thus Arabidopsis mitochondria contain a protein with high sequence identity to a plastid protein import receptor. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.