Denatonium benzoate bitter taste perception in chronic rhinosinusitis subgroups
Denatonium benzoate bitter taste perception in chronic rhinosinusitis subgroups
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DOI：
10.1002/alr.22687
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发表时间：
2020-09-03
影响因子：
6.4
通讯作者：
Cohen, Noam A.
中科院分区：
文献类型：
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作者：
Civantos, Alyssa M.;Maina, Ivy W.;Cohen, Noam A.
慢性鼻窦炎（CRS）伴鼻息肉（CRSwNP）和CRS不伴鼻息肉（CRSsNP）以及阿司匹林加重的呼吸道疾病（AERD）具有不同程度的炎症和疾病严重程度。孤立的化学感受细胞（SCC）在鼻息肉中富集，是上呼吸道中白细胞介素25（IL-25）的主要来源，导致2型炎症，并被具有苦味的苯甲酸地那铵（DB）激活。因此，我们试图在一系列浓度下评价DB味觉，以鉴定最能区分CRS亚组与对照的1。方法CRSsNP（n = 25）、CRSwNP（n = 26）和AERD（n = 27）患者以及对照组（n = 25）以固定的随机顺序品尝6种DB浓度，在0（无强度）至12（极强度）的类别量表上评级。用和不用地那铵处理鼻窦上皮培养物，并通过流式细胞术分析IL-25。结果CRSsNP患者在所有浓度下对DB的评分均显著低于对照组：5.62 x 10（-9）M，1.00 x 10（-8）M，1.78 x 10（-8）M，3.16 x 10（-8）M，5.62 x 10（-8）M和1.00 x 10（-7）M（allp< 0.0083）。CRSwNP患者与对照组无显著差异。在1.00 x 10（-8）M和3.16 x 10（-8）M浓度下，AERD患者对DB的评分明显高于对照组（p< 0.0083）。体外数据表明，与对照组（n = 5）相比，地那铵刺激后IL-25阳性细胞（n = 5）显著增加（p= 0.012）。结论我们的研究结果将鼻窦组织的体外DB刺激与IL-25增加联系起来，并显示CRS亚组相对于对照组的DB味觉差异，CRSsNP是低敏感的，AERD是高敏感的。我们建议浓度为3.16 x 10（-8）M，用于未来的临床实用性研究。
Background Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) and CRS without nasal polyps (CRSsNP), and aspirin-exacerbated respiratory disease (AERD) have varying levels of inflammation and disease severity. Solitary chemosensory cells (SCCs) are enriched in nasal polyps, are the primary source of interleukin 25 (IL-25) in upper airways, leading to type 2 inflammation, and are activated by bitter-tasting denatonium benzoate (DB). Thus, we sought to evaluate DB taste perception at a range of concentrations in order to identify 1 that most differentiates CRS subgroups from controls. Methods CRSsNP (n = 25), CRSwNP (n = 26), and AERD (n = 27) patients as well as controls (n = 25) tasted 6 DB concentrations in a fixed, random order, rating on a category scale of 0 (no intensity) to 12 (extremely intense). Sinonasal epithelial cultures were treated with and without denatonium and analyzed for IL-25 via flow cytometry. Results CRSsNP patients rated DB as significantly less intense than did controls at all concentrations: 5.62 x 10(-9)M, 1.00 x 10(-8)M, 1.78 x 10(-8)M, 3.16 x 10(-8)M, 5.62 x 10(-8)M, and 1.00 x 10(-7)M (allp< 0.0083). CRSwNP patients did not show significant differences from controls. AERD patients rated DB as significantly more intense than did controls at concentrations of 1.00 x 10(-8)M and 3.16 x 10(-8)M (p< 0.0083). In vitro data demonstrated significant increase in IL-25-positive cells after denatonium stimulation (n = 5), compared to control (n = 5) (p= 0.012). Conclusion Our findings link in vitro DB stimulation of sinonasal tissue with increased IL-25 and show differential DB taste perception in CRS subgroups relative to the control group, with CRSsNP being hyposensitive and AERD being hypersensitive. We propose a concentration of 3.16 x 10(-8)M for future study of clinical utility.