Specificity of two absorbed ELISAs for surveys of Mycobacterium paratuberculosis in cattle.
Specificity of two absorbed ELISAs for surveys of Mycobacterium paratuberculosis in cattle.
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两种吸收 ELISA 用于牛副结核分枝杆菌调查的特异性。
DOI：
10.1111/j.1751-0813.1998.tb10193.x
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发表时间：
1998
影响因子：
1.1
通讯作者：
P. Martin
中科院分区：
文献类型：
--
作者：
T. Ellis;B. A. Carson;M. Kalkhoven;P. Martin
从西澳大利亚的7292头牛中采集血清样本[日期未给出]，并使用CSL ELISA进行检测。同时培养粪便检测m。副结核。未发现阳性样本。然后用血清评价2种吸附elisa （CSL和EMAI，商用检测试剂盒）的特异性。两种酶联免疫吸附试验均按照说明进行。汇总结果列于表中。首次检测后，CSL ELISA特异性为99.7%，EMAI ELISA特异性为96.2%，但进一步检测、与参考值比较及统计分析结果均无显著差异。
Serum samples were collected from 7292 cattle in Western Australia [date not given] and tested by CSL ELISA. Faeces were also cultured for detection ofM. paratuberculosis. No positive samples were found. The serum was then used to evaluate the specificity of 2 absorbed ELISAs (CSL and EMAI, commercial test kits). Both ELISAs were run according to instructions given. Summarized results are tabulated. CSL ELISA had a specificity of 99.7% and EMAI ELISA of 96.2% after the first tests, but further testing and a comparison of results with reference values and statistical analyses showed there to be no significant differences between the tests.