Position-dependent changes in phycobilisome abundance in multicellular cyanobacterial filaments revealed by Raman spectral analysis
Position-dependent changes in phycobilisome abundance in multicellular cyanobacterial filaments revealed by Raman spectral analysis
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拉曼光谱分析揭示多细胞蓝藻丝中藻胆体丰度的位置依赖性变化
DOI：
10.1101/2023.02.14.528583
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发表时间：
2023
影响因子：
--
通讯作者：
Imai Yuto
中科院分区：
文献类型：
--
作者：
Ishihara Jun-ichi;Imai Yuto
一维丝状蓝藻--鱼腥藻。PCC7120显示了一个简单的形态模式，在缺氮条件下由两种不同的细胞类型组成。我们发现，利用拉曼显微镜可以区分分化细胞(异形胞)和未分化细胞(营养细胞)中的微生物色素成分。营养细胞中藻蓝蛋白和别藻蓝蛋白的拉曼光谱强度高于异形胞。然而，在远离异形囊的营养细胞中，这些带的强度在统计学上较低。也就是说，单个细胞中的色素成分受到细丝中的位置信息的影响。
The one-dimensional filamentous cyanobacterium, Anabaena sp. PCC 7120, shows a simple morphological pattern consisting of two distinct cell types under nitrogen-deprived conditions. We found that microbial pigment composition in differentiated (heterocyst) and undifferentiated cells (vegetative cells) can be distinguished using Raman microscopy. The Raman bands associated with phycocyanin and allophycocyanin were of higher intensity in vegetative cells than those in heterocysts. However, these bands had statistically lower intensity in vegetative cells located further away from heterocysts. That is, the pigment composition in individual cells is affected by locational information in a filament.