Two glial cell lineages diverge prenatally in rat optic nerve.
Two glial cell lineages diverge prenatally in rat optic nerve.
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DOI：
10.1016/0012-1606(84)90060-5
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发表时间：
1984-11
影响因子：
2.7
通讯作者：
M. Raff;E. Abney;Rob Miller
中科院分区：
文献类型：
--
作者：
M. Raff;E. Abney;Rob Miller
以前在新生大鼠视神经培养物中已经描述了三种类型的神经胶质细胞--少突胶质细胞、1型星形胶质细胞和2型星形胶质细胞--它们可以用三种不同的抗体来区分：抗半乳糖苷酶抗体识别少突胶质细胞;抗神经胶质细胞酸性蛋白的抗体识别两种类型的星形胶质细胞，而A2 B5单克隆抗体区分这两种类型，与2型而不是1型星形胶质细胞结合。随后显示，少突胶质细胞和2型星形胶质细胞，而不是1型星形胶质细胞，在7天视神经培养物中从共同的A2 B5+祖细胞发育。在本研究中，大鼠神经抗原-2（Ran-2），一种由单克隆抗体定义的细胞表面抗原，在视神经细胞上的分布进行了研究。结果表明，与A2 B5相反，Ran-2存在于视神经培养物中的1型而非2型星形胶质细胞上。更重要的是，研究表明Ran-2和A2 B5抗体与胚胎第17天（E17）和出生后第1天（P1）视神经细胞悬液中基本上不重叠的细胞群反应，Ran-2+，A2 B5 −群包含1型星形胶质细胞及其前体，而A2 B5+，Ran-2−群包含少突胶质细胞和2型星形胶质细胞的祖细胞。这些研究结果提供了强有力的证据表明，大鼠视神经的胶质细胞发展为两个不同的lineages-one引起1型星形胶质细胞和其他少突胶质细胞和2型星形胶质细胞，这两个谱系分歧早在E17。
Three types of glial cells have been previously described in cultures of neonatal rat optic nerve—oligodendrocytes, type 1 astrocytes, and type 2 astrocytes—which can be distinguished using three different antibodies: antigalactocerebroside antibodies recognize oligodendrocytes; antibodies against glial fibrillary acidic protein recognize both types of astrocytes, while the A2B5 monoclonal antibody distinguishes between the two, binding to type 2 but not type 1 astrocytes. It was subsequently shown that oligodendrocytes and type 2 astrocytes, but not type 1 astrocytes, develop in cultures of 7 day optic nerve from a common, A2B5+progenitor cell. In the present study, the distribution of rat neural antigen-2 (Ran-2), a cell-surface antigen defined by a monoclonal antibody, has been examined on optic nerve cells. It is demonstrated that, in contrast to A2B5, Ran-2 is present on type 1 but not type 2 astrocytes in optic nerve cultures. More importantly, it is shown that Ran-2 and A2B5 antibodies react with largely nonoverlapping populations of cells in cell suspensions of embryonic Day 17 (E17) and postnatal Day 1 (P1) optic nerve, and that the Ran-2+, A2B5−population contains type 1 astrocytes and their precursors while the A2B5+, Ran-2−population contains the progenitor cells for oligodendrocytes and type 2 astrocytes. These findings provide strong evidence that the glial cells of the rat optic nerve develop as two distinct lineages—one giving rise to type 1 astrocytes and the other to oligodendrocytes and type 2 astrocytes—and that the two lineages diverge as early as E17.