IL-7 receptor expression levels do not identify CD8+ memory T lymphocyte precursors following peptide immunization
IL-7 receptor expression levels do not identify CD8+ memory T lymphocyte precursors following peptide immunization
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DOI：
10.4049/jimmunol.175.7.4400
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发表时间：
2005-10-01
影响因子：
4.4
通讯作者：
Labrecque, N
中科院分区：
文献类型：
--
作者：
Lacombe, MH;Hardy, MP;Labrecque, N
识别效应T细胞存活及其分化为记忆T淋巴细胞的机制对于理解记忆发育至关重要。由于细胞因子调节T淋巴细胞的增殖、分化和存活，我们假设细胞因子信号传导决定效应T细胞的命运。为了跟踪T细胞应答期间的细胞因子受体表达，我们将鼠TCR转基因T细胞转移到幼稚受体中，然后用在佐剂中乳化或在树突状细胞上脉冲的肽免疫。我们的发现并没有将效应CD 8（+）细胞上IL-7 R α链和IL-2 R β链的表达与记忆T淋巴细胞的产生相关联。然而，我们可以将效应T细胞上IL-7 R α表达下调的程度与免疫产生的炎症水平相关联。此外，我们的研究结果表明，维持高水平的IL-7 R表达的效应T细胞在高峰期的反应并不排除他们的死亡。这表明维持IL-7 R表达不足以阻止T细胞收缩。因此，我们的研究结果表明，IL-7 R的表达并不总是一个很好的标志物，用于识别记忆T细胞的前体效应和IL-7 R的效应T细胞的选择性表达不应该被用来预测疫苗接种的成功。
Identification of the mechanisms underlying the survival of effector T cells and their differentiation into memory T lymphocytes are critically important to understanding memory development. Because cytokines regulate proliferation, differentiation, and survival of T lymphocytes, we hypothesized that cytokine signaling dictates the fate of effector T cells. To follow cytokine receptor expression during T cell responses, we transferred murine TCR transgenic T cells into naive recipients followed by immunization with peptide emulsified in adjuvant or pulsed on dendritic cells. Our findings did not correlate IL-7R alpha-chain and IL-2R beta-chain expression on effector CD8(+) cells with the generation of memory T lymphocytes. However, we could correlate the extent of IL-7R alpha expression down-regulation on effector T cells with the level of inflammation generated by the immunization. Furthermore, our findings showed that the maintenance of a high level of IL-7R expression by effector T cells at the peak of the response does not preclude their death. This suggests that maintenance of IL-7R expression is not sufficient to prevent T cell contraction. Thus, our results indicate that expression of the IL-7R is not always a good marker for identifying precursors of memory T cells among effectors and that selective expression of the IL-7R by effector T cells should not be used to predict the success of vaccination.