Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat.
Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat.
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DOI：
10.1186/1944-3277-10-7
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发表时间：
2015
影响因子：
--
通讯作者：
Spring S
中科院分区：
文献类型：
--
作者：
Ben Hania W;Joseph M;Schumann P;Bunk B;Fiebig A;Spröer C;Klenk HP;Fardeau ML;Spring S
在对基里巴斯环礁（基里巴斯共和国，中太平洋）21号湖的石化高盐微生物垫的厌氧微生物群落的研究期间，分离出菌株L21-RPul-D2 T。最接近的系统发育邻居是非洲螺旋体Z-7692 T，其与新菌株共享90%的16 S rRNA基因序列同一性值，因此仅为远亲。一个全面的多相研究，包括确定完整的基因组序列，开始表征新的分离。菌株L21-RPul-D2 T的细胞大小为0.2 - 0.25 × 8-9 μm，呈螺旋状，能运动，革兰氏染色阴性，产生类胡萝卜素样橙子色素。生长的最佳条件是35°C，50 g/l NaCl的盐度和约7.0的pH。生长的优选底物是碳水化合物和一些羧酸。该新菌株具有专性发酵代谢，在葡萄糖上生长期间产生乙醇、乙酸盐、乳酸盐、氢气和二氧化碳。菌株L21-RPul-D2 T是耐氧的，但氧不刺激生长。主要细胞脂肪酸为C14：0、iso-C15：0、C16：0和C18：0。主要极性脂质为一种未鉴别的氨基脂质、磷脂酰甘油、一种未鉴别的磷脂和两种未鉴别的糖脂。全细胞水解产物含有L-鸟氨酸作为细胞壁肽聚糖的诊断性二氨基酸。确定并注释了完整的基因组序列。该基因组由一条环状染色体组成，大小为3.78Mbp，包含3450个蛋白质编码基因和50个RNA基因，其中包括2个核糖体RNA基因操纵子。从基因组序列确定DNA G + C含量为51.9mol%。没有预测的基因编码细胞色素或酶负责呼吸脂醌的生物合成。根据与未培养的螺旋体属模式种之间的显著差异，将S. plicatilis，以及任何其他与细菌遗传学相关的培养物种，建议将菌株L21-RPul-D2 T（=DSM 27196 T = JCM 18663 T）置于新的种和属中，其名称为太平洋盐旋藻属新种，sp. nov.的报道。
During a study of the anaerobic microbial community of a lithifying hypersaline microbial mat of Lake 21 on the Kiritimati atoll (Kiribati Republic, Central Pacific) strain L21-RPul-D2T was isolated. The closest phylogenetic neighbor was Spirochaeta africana Z-7692T that shared a 16S rRNA gene sequence identity value of 90% with the novel strain and thus was only distantly related. A comprehensive polyphasic study including determination of the complete genome sequence was initiated to characterize the novel isolate. Cells of strain L21-RPul-D2T had a size of 0.2 – 0.25 × 8–9 μm, were helical, motile, stained Gram-negative and produced an orange carotenoid-like pigment. Optimal conditions for growth were 35°C, a salinity of 50 g/l NaCl and a pH around 7.0. Preferred substrates for growth were carbohydrates and a few carboxylic acids. The novel strain had an obligate fermentative metabolism and produced ethanol, acetate, lactate, hydrogen and carbon dioxide during growth on glucose. Strain L21-RPul-D2T was aerotolerant, but oxygen did not stimulate growth. Major cellular fatty acids were C14:0, iso-C15:0, C16:0 and C18:0. The major polar lipids were an unidentified aminolipid, phosphatidylglycerol, an unidentified phospholipid and two unidentified glycolipids. Whole-cell hydrolysates contained L-ornithine as diagnostic diamino acid of the cell wall peptidoglycan. The complete genome sequence was determined and annotated. The genome comprised one circular chromosome with a size of 3.78 Mbp that contained 3450 protein-coding genes and 50 RNA genes, including 2 operons of ribosomal RNA genes. The DNA G + C content was determined from the genome sequence as 51.9 mol%. There were no predicted genes encoding cytochromes or enzymes responsible for the biosynthesis of respiratory lipoquinones. Based on significant differences to the uncultured type species of the genus Spirochaeta, S. plicatilis, as well as to any other phylogenetically related cultured species it is suggested to place strain L21-RPul-D2T (=DSM 27196T = JCM 18663T) in a novel species and genus, for which the name Salinispira pacifica gen. nov., sp. nov. is proposed.