The neural plate specifies somite size in the Xenopus laevis gastrula
The neural plate specifies somite size in the Xenopus laevis gastrula
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DOI：
10.1016/s1534-5807(01)00018-1
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发表时间：
2001-07-01
影响因子：
11.8
通讯作者：
Harland, RM
中科院分区：
文献类型：
--
作者：
Mariani, FV;Choi, GB;Harland, RM
传统上，组织者被认为是体节诱导信号的主要来源。我们在这里表明，来自神经板的信号指定体节组织并调节爪蟾原肠胚的体节大小。异位未分化神经组织诱导大量体节扩张，但牺牲了中间板和侧板中胚层。尽管早期扩展的体节表达肌肉特异性标记，但只有一部分最终分化，这表明肌节的发育需要额外的信号。外植体测定表明，即使在组织者不存在的情况下，神经组织也会诱导体节特异性标记物的表达。最后，我们证明神经组织是适当体节发育所必需的，因为消除神经前体会导致体节明显减少。因此，体节和神经组织之间存在重要的相互作用，这种相互作用是相互加强的，对于正常胚胎模式至关重要。
The organizer has traditionally been considered the major source of somite-inducing signals. We show here that signaling from the neural plate specifies somite tissue and regulates somite size in the Xenopus gastrula. Ectopic undifferentiated neural tissue induces massive somite expansion at the expense of intermediate and lateral plate mesoderm. Although the early expanded somite expresses muscle-specific markers, only a portion terminally differentiates, suggesting that myotome development requires additional signals. Explant assays demonstrate that neural tissue induces somite-specific marker expression even in the absence of the organizer. Finally, we demonstrate that neural tissue is required for proper somite development because elimination of neural precursors results in pronounced somite reduction. Thus, an important reciprocal interaction exists between somite and neural tissue that is mutually reinforcing and critical for normal embryonic patterning.