A new screening method for proteinuria using Erythrosin B and an automated analyzer-Rapid, sensitive and inexpensive determination
A new screening method for proteinuria using Erythrosin B and an automated analyzer-Rapid, sensitive and inexpensive determination
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DOI：
10.1016/j.cca.2012.02.029
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发表时间：
2012-07-11
影响因子：
5
通讯作者：
Tomino, Yasuhiko
中科院分区：
文献类型：
--
作者：
Horikoshi, Satoshi;Higurashi, Asami;Tomino, Yasuhiko
背景：尿蛋白筛查对肾脏疾病的早期诊断已迫在眉睫，但目前还没有一种快速、准确、经济的检测方法。方法：在尿样中加入含有缓冲剂(pH 2.3)和表面活性物质的溶液和红红素B溶液。结果：人血清白蛋白浓度在2.4~2 0 0 mg/L范围内与浓度呈良好的线性关系，检出限为2.4 mg/L，比传统的染料结合法高一个数量级，与比浊法相当。对70例蛋白尿纸片筛查试验呈(-)或(+/-)阳性的患者和79例健康志愿者的现场尿样进行分析。结论：该方法是一种可行的尿蛋白免疫分析方法，可用于早期肾脏疾病的诊断。(C)2012爱思唯尔B.V.保留所有权利。
Background: In spite of the urgent necessity for a screening test of urinary protein for the early diagnosis of kidney diseases, a rapid, accurate and cost-effective method for their detection has yet to be developed.Methods: A solution containing a buffer agent (pH 2.3) and surfactants and a solution of Erythrosin B are added to a urine sample. After letting the mixture stand for 5 min at 37 degrees C, the dye-bound protein is measured by a spectrophotometer at 546 nm using a Hitachi 7170S automated analyzer.Results: The calibration curve was linear with human serum albumin concentration in the range of 2.4-200 mg/l. The detection limit, 2.4 mg/l was superior to conventional dye-binding methods by one order of magnitude and comparable to the turbidimetric immunoassay (TIA). Spot urine samples from 70 patients who showed (-) or (+/-) in the dip-stick screening test for proteinuria and 79 healthy volunteers were analyzed. There was an excellent correlation (r = 0.978, n = 149) between the results given by the proposed method and those by the TIA.Conclusions: This method provides a viable alternative to the conventional immunoassay-based methods for urinary protein measurement, and will be useful in the diagnosis of early stage kidney disease. (C) 2012 Elsevier B.V. All rights reserved.