Odor-Induced Persistent Discharge of Mitral Cells in the Mouse Olfactory Bulb
Odor-Induced Persistent Discharge of Mitral Cells in the Mouse Olfactory Bulb
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DOI：
10.1152/jn.91019.2008
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发表时间：
2009-04-01
影响因子：
2.5
通讯作者：
Mori, Kensaku
中科院分区：
文献类型：
--
作者：
Matsumoto, Hideyuki;Kashiwadani, Hideki;Mori, Kensaku
松本H，柏谷H，长尾H，Aiba A，Mori K.Odo诱导小鼠嗅球二尖瓣细胞持续放电。《神经生理学杂志》101：1890-1900,2009。2009年1月21日首次出版；DOI：10.1152/jn.91019.2008。感觉信息以中枢神经元持续活动的形式短期保持，在将短暂的感觉刺激转变为长期的大脑反应方面起着关键作用。嗅觉系统将这种转换用于各种功能目的，但潜在的神经机制仍然难以捉摸。在这里，我们记录了乌拉坦麻醉下小鼠嗅球(OB)中二尖瓣和簇状(M/T)细胞的气味诱发的单一单位棘波反应，并研究了M/T细胞持续放电(PD)持续数十秒的神经机制。气味刺激后产生的持续性后放电在幅度、气味特异性和气味浓度-反应关系方面与气味诱导的即刻尖峰反应的特征不同。这表明，嗅觉感觉神经元的长时间输入以外的神经机制参与了这些后放电的产生。代谢型谷氨酸受体1(MGluR1)表达于M/T细胞的树突状细胞，被认为参与了M/T细胞之间的肾小球内相互作用。在缺乏mGluR1或局部使用mGluR1选择性拮抗剂的OBS中，气味诱发的棘波反应的持续时间显著低于对照组，表明球神经元回路中的mGluR1参与了PD的产生。这些结果表明，OB的神经元回路可以积极地延长气味诱导的球输出神经元的尖峰活动，从而将短暂的气味输入转化为中枢嗅觉系统中的长期活动。
Matsumoto H, Kashiwadani H, Nagao H, Aiba A, Mori K. Odorinduced persistent discharge of mitral cells in the mouse olfactory bulb. J Neurophysiol 101: 1890-1900, 2009. First published January 21, 2009; doi: 10.1152/jn.91019.2008. Short-term retention of sensory information in the form of persistent activity of central neurons plays a key role in transforming a brief sensory stimulation into longer-lasting brain responses. The olfactory system uses this transformation for various functional purposes, but the underlying neuronal mechanisms remain elusive. Here, we recorded odor-evoked, single-unit spike responses of mitral and tufted (M/ T) cells in the mouse olfactory bulb (OB) under urethane anesthesia and examined the neuronal mechanisms of the persistent discharge (PD) of M/ T cells that outlasts the odor stimulus for tens of seconds. The properties of the persistent afterdischarge that occurred after odor stimulation were distinct from those of odor-induced immediate spike responses in terms of the magnitude, odorant specificity, and odorant concentration-response relationship. This suggests that neuronal mechanisms other than prolonged input from olfactory sensory neurons are involved in generating these afterdischarges. Metabotropic glutamate receptor 1 (mGluR1) is expressed in the dendrites of M/ T cells and is thought to participate in intraglomerular interactions among M/ T cells. In OBs lacking mGluR1, or treated locally with an mGluR1-selective antagonist, the duration of the odor-induced spike responses was significantly lower than that in control OBs, indicating that mGluR1 within the bulbar neuronal circuits participates in the PD generation. These results suggest that neuronal circuits in the OB can actively prolong the odor-induced spike activity of bulbar output neurons and thus transform a brief odor input into longer-lasting activity in the central olfactory system.