Immunohistochemical localization of phosphodiesterase 10A in multiple mammalian species
Immunohistochemical localization of phosphodiesterase 10A in multiple mammalian species
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DOI：
10.1369/jhc.6a6930.2006
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发表时间：
2006-11-01
影响因子：
3.2
通讯作者：
Stephenson, Diane T.
中科院分区：
文献类型：
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作者：
Coskran, Timothy M.;Morton, Daniel;Stephenson, Diane T.
利用一种靶向大鼠磷酸二酯酶10A （PDE10A）氨基末端的单克隆抗体，将PDE10A定位于小鼠、大鼠、狗、食蟹猴和人的多个中枢神经系统和外周组织。PDE10A免疫反应性在这些物种的中枢神经系统中强烈表达，在外周组织中表达有限。在大脑中，在纹状体的神经元细胞体和神经元中，在纹状体和纹状体白质轨道中，以及在黑质和苍白球中，都存在强烈的免疫反应。在脑外，PDE10A的免疫反应性较弱，分布仅限于少数组织，如睾丸、附睾精子和肠神经节。这些数据表明，PDE10A是一种进化上保守的磷酸二酯酶，在大脑中高度表达，但在多种哺乳动物的外周分布有限。
A monoclonal antibody directed against the amino terminal of rat phosphodiesterase 10A (PDE10A) was used to localize PDE10A in multiple central nervous system (CNS) and peripheral tissues from mouse, rat, dog, cynomolgus macaque, and human. PDE10A immunoreactivity is strongly expressed in the CNS of these species with limited expression in peripheral tissues. Within the brain, strong immunoreactivity is present in both neuronal cell bodies and neuropil of the striatum, in striatonigral and striatopallidal white matter tracks, and in the substantia nigra and globus pallidus. Outside the brain, PDE10A immunoreactivity is less intense, and distribution is limited to few tissues such as the testis, epididymal sperm, and enteric ganglia. These data demonstrate that PDE10A is an evolutionarily conserved phosphodiesterase highly expressed in the brain but with restricted distribution in the periphery in multiple mammalian species.