Plasmacytoid dendritic cells are present in cervical carcinoma and become activated by human papillomavirus type 16 virus-like particles
Plasmacytoid dendritic cells are present in cervical carcinoma and become activated by human papillomavirus type 16 virus-like particles
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DOI：
10.1016/j.ygyno.2004.10.040
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发表时间：
2005-03-01
影响因子：
4.7
通讯作者：
Hooijberg, E
中科院分区：
文献类型：
--
作者：
Bontkes, HJ;Ruizendaal, JJ;Hooijberg, E
目标.浆细胞样树突状细胞（PDC）通过分泌高水平的IFN α在对病毒感染的先天性免疫应答中起重要作用。我们研究PDC是否在人乳头瘤病毒（HPV）相关的宫颈癌中发挥作用。分析18例宫颈癌的冰冻切片中髓样和浆细胞样DC的存在。为了研究HPV病毒是否可以激活PDC，分析了健康供体外周血单核细胞中PDC上推定的VLP受体（CD 49 f和CD 16）的表达。此外，在与HPV 16病毒样颗粒（VLP）孵育后，检测了纯化的血液来源PDC对CD 83的诱导作用和IFN α的产生。在83%的CxCa病例中检测到PDC，主要在间质中。PDC表达一种假定的VLP受体（CD 49 f）。在与VLP孵育后，诱导PDC产生IFN α，但不诱导CD 83表达。我们的数据表明，PDC，这是在子宫颈局部在手，在自然免疫反应中发挥作用，对HPV和确定PDC作为可能的tat-gets为VLP为基础的疫苗。(c)2004年爱思唯尔公司All rights reserved.
Objectives. Plasmacytoid dendritic cells (PDC) play an important role in the innate immune response to viral infections through the secretion of high levels of IFN alpha. We investigated whether PDC play a role in Human Papillomavirus (HPV) associated cervical carcinoma.Methods. Frozen sections of 18 cervical carcinomas were analyzed for the presence of myeloid and plasmacytoid DC. To study whether the HPV virus can activate PDC, expression of putative VLP receptors (CD49f and CD16) was analyzed on PDC in peripheral blood mononuclear cells of healthy donors. Furthermore, CD83 induction and IFNa production by purified blood-derived PDC was measured after incubation with HPV 16 virus like particles (VLP).Results. PDC were detected in 83% of the CxCa cases, primarily in the stroma. PDC express one of the putative VLP receptors (CD49f). IFNa production but no CD83 expression was induced in PDC upon incubation with VLP.Conclusion. Our data suggest that PDC, which are at hand locally in the cervix, play a role in the natural immune response against HPV and identify PDC as possible tat-gets for VLP-based vaccines. (c) 2004 Elsevier Inc. All rights reserved.